FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016908
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATTACTGTGCGAGATTGGATCGGTCAGATAAAGTGGGGGCTATTCC32720.3217596872086757No Hit
GTAATAAACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA32100.31566277382024727No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22190.21821049691810862No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC20050.19716631199675883No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT19250.18929932697943178No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18990.18674255684880048No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18500.18192402852568768No Hit
GTGTTACTTGTGGGGGAAACAACATTGAAAGGAAAAGTGTGCACTGGTAC18070.1776955240788744No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17910.17612212707540897No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16940.16658340774189995No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16170.1590114346627227No Hit
ATTCCGGACTGGGGCCAGGGAACCCTGGTCATCGTCTCCTCAGCCTCCAC15140.14888269145291413No Hit
ACCATAAAGTACGCAGACTCTGTGAAGGGGCGATTCACCATCTCCAGAGA15070.14819433026389803No Hit
CTAGTAGTATCATACACCTGACAGAAATAGTCGGCCTCATCCCCGGCTTC15010.14760430638759847No Hit
ATCATACACCTGACAGAAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC14500.1425891034390525No Hit
GTGCTGGGTTTTCCTTGTTGCTTTTTTAGAAGGTGTCCAGTGTGAGGTGC14360.14121238106102027No Hit
CCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG14340.14101570643558708No Hit
TACTACTAGTGATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC14290.14052401987200416No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTA13750.1352138049853084No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA13650.1342304318581425No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13630.13403375723270936No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG13360.13137864978936148No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13340.1311819751639283No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT13270.1304936139749122No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA13260.1303952766621956No Hit
GTATTACTGGTAGTACCATAAAGTACGCAGACTCTGTGAAGGGGCGATTC13220.13000192741132927No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12920.12705180802983163No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGA12640.12429836327376716No Hit
GTGTATGATACTACTAGTGATCATTGGGTGTTCGGCGGAGGGACCAAGCT12480.12272496627030174No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12330.12124990657955291No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11920.1172180767581728No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11850.11652971556915669No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC11730.11534966781655764No Hit
GTTCTGGTCATGTCTTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG11710.11515299319112447No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11310.11121950068246095No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT11260.110727814118878No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10930.10748268279923061No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA10780.10600762310848177No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCTTCA10540.10364752760328368No Hit
GTCTTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT10530.1035491902905671No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA10510.10335251566513393No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10310.10138576941080217No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG10260.10089408284721922No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG10220.10050073359635288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13450.065.789431
TCAACGC13850.062.2880864
ATCAACG13900.061.546833
TATCAAC14750.058.0114972
CAACGCA15050.057.796435
GGTATCA5950.055.617841
AACGCAG16300.054.224866
CGCAGAG17650.049.770748
GCAGAGT22350.038.9822469
GTACATG26800.038.3861471
ACATGGG26350.038.196273
TACATGG26900.037.4226842
CATGGGA14700.036.6789974
ATGACCG1001.462685E-435.9436455
GAATACG1854.472895E-935.0291639
TATTGAG12700.034.528545
ATGGGAG14500.033.2168855
TTATTGA14000.030.8103584
GTACACG11000.030.7154775
GTATGTA13800.028.6719261
Produced by FastQC (version 0.11.5)