FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841773
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATTACTGTGCGAGACATTACTATGATAGTGCGGGTCATTACCCAGA37960.450952929115094No Hit
GTAATATATAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGAGA33720.4005830550516588No Hit
GTCTCTGGTGGCTCCATCACCAGTAATGATCACTATTGGGGCTACATCCG27570.3275229782851196No Hit
CTATTGGGGCTACATCCGCCAGCCCCCCGGGAAGGAACTGGAGTGGATTG15920.18912462148346407No Hit
ATCATAGTAATGTCTCGCACAGTAATATATAGCCGTGTCTGCGGCGGTCA14740.17510659049411184No Hit
GCTATATATTACTGTGCGAGACATTACTATGATAGTGCGGGTCATTACCC14250.16928554372734692No Hit
ATATTACTGTGCGAGACATTACTATGATAGTGCGGGTCATTACCCAGACT14040.1667908093987334No Hit
TACTATGATAGTGCGGGTCATTACCCAGACTATTGGGGCCAGGGAACCCT13220.1570494658298615No Hit
GTCCCTAACCTGCACTGTCTCTGGTGGCTCCATCACCAGTAATGATCACT12930.153604356518919No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.1531291690277545No Hit
AACCTGCACTGTCTCTGGTGGCTCCATCACCAGTAATGATCACTATTGGG12610.14980285658960313No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCT12600.14968405971681203No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCC12550.1490900753528564No Hit
ATATATAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGAGAAAT12230.14528857542354057No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12220.14516977855074945No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11980.14231865360376253No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10870.12913220072394815No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10630.12628107577696127No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCCTGTCCGTTGACACGTCC10470.12438032581230331No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10340.12283596646601874No Hit
CAGTAATATATAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGA10080.11974724777344961No Hit
TCCTCACCCTCATCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG10030.119153263409494No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10020.1190344665367029No Hit
ATACCACACCATTCAGACCGATGTCCCATGCTGCACAGAAATAATCAGCC9710.11535176348017814No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9560.11356981038831133No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC9240.10976831045899549No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA9070.10774876362154642No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG8920.10596681052967961No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT8710.10347207620106608No Hit
CTATGATAGTGCGGGTCATTACCCAGACTATTGGGGCCAGGGAACCCTGG8540.10145252936361704No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8450.10038335750849695No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16150.088.891
ATCAACG20250.070.1507953
TCAACGC20300.069.973854
TATCAAC20550.069.828492
GGTATCA5800.068.408021
CAACGCA21800.065.482095
AACGCAG22600.063.3888666
ACGCAGA24900.057.812487
CGCAGAG25200.057.1242338
GCAGAGT29050.049.3057869
TATACAG5150.032.1984145
CTGTGCG19050.031.3598569
TACATGG22250.028.519642
ATATTAC21650.027.6446823
GTACATG22800.027.5268651
TATTACT22950.027.3339334
TATATTA22600.026.8016952
CAGAGTA27900.026.5719819
TACTGTG24750.026.46417
TTACTGT23750.025.4615026
Produced by FastQC (version 0.11.5)