FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283740
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTC46670.36354713571283903No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC35360.2754451836041566No Hit
GTCTACATGGAGTTGAGTAGCCTGAATTTTGAAGACACGGCCGTTTATTA26300.2048701450449468No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25700.2001963014317541No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATGTCTTATAAGTACCA24900.19396450994749714No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23880.18601897580506957No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATGTCTT22530.175502827675386No Hit
TCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGT20610.16054652811316933No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19480.1517441226416564No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA18300.14255223020237742No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT17990.14013741100222785No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17860.13912474488603613No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC16960.13211397946624706No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16250.126583264523969No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA16030.12486952186579836No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15670.12206521569788276No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG15300.11918301213641391No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14890.11598921900073224No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATGTCTTATAAGTACCAC14790.11521024506520011No Hit
GTAGTAATCAGCCTCATCCTCCACCTGGGCCCCAGTGATAGTCAAGGTGG14750.11489865549098727No Hit
CCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCAGGACAAACG14590.11365229719413589No Hit
CCCCTGTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCT14230.11084799102622026No Hit
TTACTACTGTTACTCAACAGACAGCAGTGGTACTTATAAGACATTTGGCG13750.1071089161356661No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13700.10671942916790005No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13660.1064078395936872No Hit
GATTACTACTGTTACTCAACAGACAGCAGTGGTACTTATAAGACATTTGG13650.10632994220013399No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13560.10562886565815507No Hit
GTCTGGGGCTGAGGTTAAGAAGCCAGGGGCCTCAGTGAACATTTCCTGTA13440.10469409693551655No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG13430.10461619954196333No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA13370.10414881518064406No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13350.10399302039353762No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA13120.10220138034181375No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13110.10212348294826054No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12930.10072132986430275No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29050.064.7313461
TCAACGC34350.054.2966844
ATCAACG34700.053.5414963
CAACGCA35350.052.7545435
TATCAAC36250.051.2521362
AACGCAG36700.051.0081866
CGCAGAG41100.045.545688
ACGCAGA45450.041.0281147
GCAGAGT47350.039.5338489
GAATACG3900.035.0755659
GTGCTAG5350.035.013851
GTATACG900.003741518432.0212141
GTACATG37150.031.2239131
ACATGGG36850.029.7034153
CGTAACA1000.00627919228.8044952
GTACACG8300.028.6276285
ATATACG6100.028.3466471
ATACGAG5900.028.0721783
CTAGAGA6450.027.9113334
TACACGG8700.027.3103526
Produced by FastQC (version 0.11.5)