FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283740
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTC41280.3215604405876579No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC30070.23423746241450763No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23660.1843052331468989No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATGTCTTATAAGTACCA22290.1736332902301089No Hit
GTCTACATGGAGTTGAGTAGCCTGAATTTTGAAGACACGGCCGTTTATTA21870.170361599700874No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATGTCTT20310.158209606306573No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20240.15766432455170049No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18400.14333120413790953No Hit
TCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGT18020.1403711031828875No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16570.1290759811176718No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA16120.1255705984077773No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT15860.12354526617539376No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC14930.11630080857494508No Hit
CCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCAGGACAAACG14220.11077009363266706No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13920.1084331718260707No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13770.10726471092277254No Hit
CCCCTGTGCTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCT13560.10562886565815507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13410.1044604047548569No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13290.10352563603221836No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG13190.10274666209668624No Hit
GTAGTAATCAGCCTCATCCTCCACCTGGGCCCCAGTGATAGTCAAGGTGG13160.10251296991602663No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13100.10204558555470734No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13030.10150030379983487No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27150.071.861321
TCAACGC35200.053.7992824
ATCAACG35500.053.344643
TATCAAC37050.051.501642
CAACGCA39800.047.7621885
AACGCAG40800.046.591556
CGCAGAG42750.043.792598
ACGCAGA45500.041.1457867
GCAGAGT47450.038.8402989
GAATACG3750.038.3952529
GTGCTAG4700.035.231061
TCCGCTA4250.032.1905258
CAGAGTA45650.032.171279
TATTGAG19300.030.592855
GCGTTAC950.00489257330.3132211
GTACATG34350.028.9233171
AATCCGC4500.028.802056
TTATTGA21150.028.5977784
TACACCG2354.595131E-827.5764315
ACATGGG35500.027.1793983
Produced by FastQC (version 0.11.5)