FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2507731
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGAGACAGCCACCCTCGGTGTCAGTGGC155690.6208401140313693No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC123980.494391144823747No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAAGACCCTATAATTGGCA87540.34908050345112773No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC85150.3395499756552836No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAAGACCCT78990.31498593748691545No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT73140.29165807656403336No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA67370.2686492291238574No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA66460.26502045075807573No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT62190.2479931061186387No Hit
ATTATAGGGTCTTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCC60740.24221098674459102No Hit
GTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGG57820.23056699462581914No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT56390.22486462862244794No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC56150.2239075881743297No Hit
GACTACTACTGTTCCTCAACAGACATCAGTGCCAATTATAGGGTCTTCGG55750.22231252076079933No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA55120.21980028958448894No Hit
CCTATGAGCTGAGACAGCCACCCTCGGTGTCAGTGGCCCCAGGACAAACG54500.21732793509351683No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54000.2153341008266038No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG50030.1995030567473146No Hit
CCCCTGTGGTGGTCATTTATGAGGACTACAAACGACCCTCCGGGATCCCT49390.19695094888566594No Hit
CCCTATAATTGGCACTGATGTCTGTTGAGGAACAGTAGTAGTCAGCTTCA48960.1952362514161208No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG48520.19348167726123733No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA48340.19276389692514867No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT48190.19216574664507477No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48070.19168722642101566No Hit
GGCCAGGCCCCTGTGGTGGTCATTTATGAGGACTACAAACGACCCTCCGG48070.19168722642101566No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG47090.18777931125786618No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC47080.18773943457252792No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG46410.1850676966548645No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG45960.1832732458146428No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA45950.18323336912930455No Hit
TTTATGAGGACTACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC44430.17717211295788904No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA44310.17669359273382992No Hit
CTCTGAGGCCTCCTATGAGCTGAGACAGCCACCCTCGGTGTCAGTGGCCC44150.17605556576841774No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA42560.1697151727996344No Hit
ACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAAGACCCTATAATTGGCAC42550.16967529611429616No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGAGACAGCCACCCTCG41820.1667642980846032No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT40860.16293613629213022No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT39800.15870920764627466No Hit
GGTCAGGCCAGGCCCCTGTGGTGGTCATTTATGAGGACTACAAACGACCC37840.1508933773199757No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAGG37130.14806213266095924No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC36740.14650694193276712No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA36290.14471249109254541No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG35480.14148247958014637No Hit
ATAGTAGACGGCCGTGTCGTCGGATCTCAGGCTGCTCAGTTGCAAGTAGA35430.14128309615345505No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC35220.14044568576135158No Hit
CCACAGGGGCCTGGCCTGACCTCTGCTGGTACCAATAAGCATATTTTTTT34340.1369365374515847No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC33440.13334763577114134No Hit
GTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCC32880.13111454139219877No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG31910.12724650291438755No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG31500.1256115588155189No Hit
GTATCAACGCAGAGTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAA31480.12553180544484238No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT30360.12106561668695724No Hit
GTCTACTTGCAACTGAGCAGCCTGAGATCCGACGACACGGCCGTCTACTA30310.12086623326026595No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC29710.11847363213997035No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28770.11472522371817392No Hit
GACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGTTCCT28340.11301052624862874No Hit
CTACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAACTCAG28190.11241237596855486No Hit
GTCTACTATTGTGCGAGGGACGTGATTGGCAGCAGTAGTTGGTTCGTGGG28010.11169459563246617No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA27780.11077743186968618No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT27250.10866396754675842No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC26680.10639099648247759No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA26270.10475605238360892No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA25980.10359962850879939No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG25870.10316098497007854No Hit
TACCAGCAGAGGTCAGGCCAGGCCCCTGTGGTGGTCATTTATGAGGACTA25810.10292172485804897No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25620.10216406783662203No Hit
CTACTACTGTTCCTCAACAGACATCAGTGCCAATTATAGGGTCTTCGGCG25620.10216406783662203No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC25540.10184505435391596No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25430.1014064108151951No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.085.460921
GTATCAA57200.078.459611
ATCAACG67550.065.766693
TCAACGC69050.064.338024
TATCAAC69600.063.8295972
CAACGCA72700.061.2068825
AACGCAG75200.059.2654656
CGCAGAG83100.053.5436178
GCAGAGT90750.049.030029
ACGCAGA91100.048.7626237
TTGAGGG26400.030.2718727
TTATTGA58100.028.9991974
GTACATG68450.028.9409891
ATTGAGG27400.028.9048716
TACATGG69350.028.4479062
ACATGGG70400.027.8190613
CAGAGTA94900.027.7673939
GTACACG13750.027.229955
TCTACAC16150.027.1959043
GGGTTTA28500.027.0453761
Produced by FastQC (version 0.11.5)