FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2507731
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGAGACAGCCACCCTCGGTGTCAGTGGC138550.5524914753615918No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC108820.43393808985094495No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAAGACCCTATAATTGGCA78860.3144675405775181No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC76880.3065719568805426No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAAGACCCT68250.27215837743362425No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT66060.26342538334454535No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA59530.2373859078186616No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA58980.23519269012505728No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT58690.23403626625024773No Hit
ATTATAGGGTCTTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCC55760.22235239744613755No Hit
GTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGG53630.2138586634690882No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA51100.2037698620785084No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT49370.19687119551498947No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48930.19511662136010602No Hit
GACTACTACTGTTCCTCAACAGACATCAGTGCCAATTATAGGGTCTTCGG48660.194039950855973No Hit
CCTATGAGCTGAGACAGCCACCCTCGGTGTCAGTGGCCCCAGGACAAACG48370.19288352698116346No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC48180.19212586995973652No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG45740.18239595873720107No Hit
CCCCTGTGGTGGTCATTTATGAGGACTACAAACGACCCTCCGGGATCCCT45620.18191743851314196No Hit
CCCTATAATTGGCACTGATGTCTGTTGAGGAACAGTAGTAGTCAGCTTCA45370.18092052137968548No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG44670.17812915340600727No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG44590.1778101399233012No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT43900.17505864863496123No Hit
GGCCAGGCCCCTGTGGTGGTCATTTATGAGGACTACAAACGACCCTCCGG43790.17462000509624037No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43710.17430099161353432No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA43710.17430099161353432No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA41940.1672428183086623No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG41340.16485021718836668No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG41070.16377354668423366No Hit
TTTATGAGGACTACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC40790.1626569994947624No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC39740.1584699475342451No Hit
ACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAAGACCCTATAATTGGCAC39720.1583901941635686No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA39520.15759266045680337No Hit
CTCTGAGGCCTCCTATGAGCTGAGACAGCCACCCTCGGTGTCAGTGGCCC39080.15583808630191992No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA38980.15543931944853734No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT38420.15320622506959478No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT37850.150933254005314No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGAGACAGCCACCCTCG37030.14766336580757666No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAGG35770.1426389034549559No Hit
GGTCAGGCCAGGCCCCTGTGGTGGTCATTTATGAGGACTACAAACGACCC34570.1378537012143647No Hit
ATAGTAGACGGCCGTGTCGTCGGATCTCAGGCTGCTCAGTTGCAAGTAGA34080.13589974363278995No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC33720.1344641829606126No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA33580.13390590936587696No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC33010.13163293830159614No Hit
GTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCC31460.12545205207416585No Hit
CCACAGGGGCCTGGCCTGACCTCTGCTGGTACCAATAAGCATATTTTTTT31450.1254121753888276No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG30560.12186315039372246No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC30220.12050734309222162No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG29640.11819449534260253No Hit
GTATCAACGCAGAGTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAA29100.1160411543343365No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG28340.11301052624862874No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT27230.10858421417608188No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27080.107986063896008No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC27030.10778668046931669No Hit
GTCTACTTGCAACTGAGCAGCCTGAGATCCGACGACACGGCCGTCTACTA26160.10431740884488806No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA25080.10001072682835599No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18850.079.077731
GTATCAA55700.077.957381
ATCAACG63250.066.937373
TCAACGC64900.065.124634
TATCAAC65950.064.4153062
CAACGCA71900.058.7842645
AACGCAG74900.056.4297526
CGCAGAG79200.052.819488
ACGCAGA87000.048.2494667
GCAGAGT87650.047.7245069
GAATACG4900.033.794739
TACATGG68150.033.069712
TTATTGA54750.033.0096934
GTACATG68500.032.483521
ACATGGG69900.032.0357633
CATGGGG50300.031.6356074
ATTGAGT38150.029.2542556
GGGTTTA27550.028.7518791
GTACACG14850.028.6073385
CAGAGTA87600.027.36899
Produced by FastQC (version 0.11.5)