FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589739
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12250.20771900790010497No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10890.1846579588597668No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9440.1600708109858768No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8350.141588058446194No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT7220.12242703975826595No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG6880.1166617774981814No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6790.11513567866462962No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT6420.10886171679336112No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14950.067.928831
ATCAACG18100.053.705773
TCAACGC18900.051.813494
TATCAAC20250.048.714842
CGTTAGT450.0099012948.0036742
CAACGCA20450.047.5342035
AACGCAG20650.047.4225166
CGCAGAG22000.044.504958
GCAGAGT25350.038.6236279
TTTCGCG800.002107240735.996659
ACGCAGA28350.034.536477
AGGACCG900.003750640232.002455
ATCCCGT1204.221966E-430.0022986
CATGGGG10500.029.4879724
GGTATCA8900.029.1332361
GTATAGG2752.2919266E-1028.8095361
GTACATG20500.028.8095321
TACATGG20150.028.230452
CAGCGCT4100.028.0997124
ACATGGG20650.027.5469043
Produced by FastQC (version 0.11.5)