FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540092
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCCTCAGATCTCAGGCTACTCAGTTCCATGTAGG15910.294579442020989No Hit
GCCCTATATTACTGTGCGAGGCTTGATTGTACTGGTGCCAGCTGCTATCA13820.2558823311583952No Hit
ATATAGGGCCGTGTCCTCAGATCTCAGGCTACTCAGTTCCATGTAGGCTG13650.25273471927005026No Hit
ATATTACTGTGCGAGGCTTGATTGTACTGGTGCCAGCTGCTATCAGCACT13540.2506980292246506No Hit
TCTATGGATCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGC12450.23051628241114477No Hit
CTATATTACTGTGCGAGGCTTGATTGTACTGGTGCCAGCTGCTATCAGCA11420.21144545744058418No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10700.1981143953252409No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA9130.16904527376817283No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT8110.15015960243810314No Hit
GCCTACATGGAACTGAGTAGCCTGAGATCTGAGGACACGGCCCTATATTA7800.1444198395828859No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7300.1351621575583419No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7230.13386608207490577No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC6930.12831147286017938No Hit
CAGTAATATAGGGCCGTGTCCTCAGATCTCAGGCTACTCAGTTCCATGTA6920.1281263192196885No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6120.11331402798041815No Hit
ACCATGGACTGGCCCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGG5980.11072187701354583No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT5900.1092406478896188No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5890.10905549424912793No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT5520.1022048095509654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9000.076.802531
CAACGCA10350.065.308565
TCAACGC10250.065.244174
ATCAACG10150.065.178513
AACGCAG11100.060.9014746
TATCAAC10950.060.416612
GGTATCA3800.054.949181
ACGCAGA12400.053.936687
CGCAGAG12950.051.6459278
TATAACG450.00995348347.939274
GCAGAGT14900.044.8868989
GTATAGG2351.3569661E-930.6393051
GTAATAT8850.030.1026861
GTCCTAC1255.3625763E-428.8009451
GTATAAC1306.7966763E-427.657273
GGGCCGT8600.027.5955099
TCTACAC4450.027.4708163
TATAGGG10350.027.0961072
AATATAG9650.026.080953
TAGGGCC9200.025.7958017
Produced by FastQC (version 0.11.5)