FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378334
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATGGCCGTGTCTTCAGATTTCAGGCTGGTCAACTCCATGTAGA35360.2565415929665814No Hit
ATGTATTACTGTGTGAGAGACCTCTATTGTTCTGGTGCTGAATGTTCGGG27710.201039806026696No Hit
GTCTACATGGAGTTGACCAGCCTGAAATCTGAAGACACGGCCATGTATTA25840.18747270255250179No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCGTCGGTGAAGGTCTCCTGCA25560.1854412645991465No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24740.1794920534500346No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT24050.17448600992212338No Hit
GTGTCGGGGTCTCCGGGGAAGACGATAACCATCTCCTGCACCGGCAGCGG24000.1741232531447385No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA23850.1730349828125839No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23120.16773873386276478No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCC22730.16490923099916277No Hit
GTATTACTGTGTGAGAGACCTCTATTGTTCTGGTGCTGAATGTTCGGGGG22580.16382096066700813No Hit
ATCAAGGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCC22490.16316799846771537No Hit
GTCTTATGACAGCAGCAATCAAGGAGTGTTCGGCGGAGGGACCAAGCTGA21660.1571462359631265No Hit
CAGTAATACATGGCCGTGTCTTCAGATTTCAGGCTGGTCAACTCCATGTA21040.15264805192355407No Hit
GTCATAAGACTGACAATAATAGTCAGCCTCGTCCTCAGTCTTCAGTCCAG20170.14633608399705733No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGAGGAT18940.13741226727338945No Hit
CTCCTTGATTGCTGCTGTCATAAGACTGACAATAATAGTCAGCCTCGTCC18900.13712206185148157No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA18410.13356704543310982No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18190.1319709156126164No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17910.12993947765926112No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17680.1282707964832907No Hit
CTATGAGGATGACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCT17630.12790803970590583No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC17620.12783548835042885No Hit
ATTTTATGCTGACTCAGCCCCGCTCTGTGTCGGGGTCTCCGGGGAAGACG17160.12449812599848803No Hit
CTATTATTGTCAGTCTTATGACAGCAGCAATCAAGGAGTGTTCGGCGGAG17010.12340985566633342No Hit
CTCCTGCACCGGCAGCGGTGGCAACATTGCCAGCAACTATGTGCAGTGGT16740.12145096906845511No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG16660.12087055822463931No Hit
GCCCCGCTCTGTGTCGGGGTCTCCGGGGAAGACGATAACCATCTCCTGCA16660.12087055822463931No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA16590.12036269873630048No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16470.1194920824705768No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16440.11927442840414587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16310.1183312607829452No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16180.11738809316174455No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16150.11717043909531362No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC16050.11644492554054386No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCGCTCTGTGT16020.11622727147411295No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16000.116082168763159No Hit
ACTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTG15960.11579196334125111No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15920.11550175791934321No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA15740.11419583352075767No Hit
ACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGC15410.11180163879001752No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA15410.11180163879001752No Hit
TTTCTGGACTGAAGACTGAGGACGAGGCTGACTATTATTGTCAGTCTTAT15200.11027806032500105No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC15140.10984275219213921No Hit
CTATTGTTCTGGTGCTGAATGTTCGGGGGCCCCCGGCTCTCAATGGGGCC15060.1092623413483234No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTCGGAGCACAGCTCAT15010.10889958457093855No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACA14980.10868193050450761No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14940.10839172508259971No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14860.10781131423878393No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14710.1067230439066293No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14650.10628773577376746No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG14620.10607008170733655No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT14500.10519946544161284No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14330.10396609239850428No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG14290.1036758869765964No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGG14290.1036758869765964No Hit
TCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGC14230.10324057884373453No Hit
ATACATGGCCGTGTCTTCAGATTTCAGGCTGGTCAACTCCATGTAGACTG14160.10273271935539571No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14080.10215230851157993No Hit
CCATGTAGACTGTGTTGGTCGACTCGTCCGCGGTAATCGTGATTCTGCCC14060.10200720580062596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)