FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378334
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATGGCCGTGTCTTCAGATTTCAGGCTGGTCAACTCCATGTAGA34570.25081003588390044No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCC26570.19276895150232093No Hit
ATGTATTACTGTGTGAGAGACCTCTATTGTTCTGGTGCTGAATGTTCGGG25980.1884884215291794No Hit
GTCTACATGGAGTTGACCAGCCTGAAATCTGAAGACACGGCCATGTATTA25590.18565891866557743No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25260.18326472393483728No Hit
GTCATAAGACTGACAATAATAGTCAGCCTCGTCCTCAGTCTTCAGTCCAG25190.18275686444649844No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCGTCGGTGAAGGTCTCCTGCA24530.17796847498501814No Hit
CTCCTTGATTGCTGCTGTCATAAGACTGACAATAATAGTCAGCCTCGTCC23860.17310753416806085No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA22650.16432882015534694No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22430.16273269033485352No Hit
GTATTACTGTGTGAGAGACCTCTATTGTTCTGGTGCTGAATGTTCGGGGG22080.16019339289315943No Hit
GTCTTATGACAGCAGCAATCAAGGAGTGTTCGGCGGAGGGACCAAGCTGA21940.15917767391648177No Hit
ATCAAGGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCC21180.15366377090023173No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT21170.15359121954475477No Hit
GTGTCGGGGTCTCCGGGGAAGACGATAACCATCTCCTGCACCGGCAGCGG19430.1409672836917612No Hit
CAGTAATACATGGCCGTGTCTTCAGATTTCAGGCTGGTCAACTCCATGTA19350.14038687284794543No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19320.1401692187815145No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17730.12863355326067558No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC17400.12623935852993542No Hit
CTATGAGGATGACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCT17180.12464322870944199No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16330.11847636349389915No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16210.11760574722817546No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA15910.11542920656386624No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15890.11528410385291228No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGAGGAT15830.11484879572005043No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15270.11078591981333986No Hit
TTTCTGGACTGAAGACTGAGGACGAGGCTGACTATTATTGTCAGTCTTAT15210.11035061168047802No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15100.10955254677023131No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA14940.10839172508259971No Hit
CAATAATAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAAATGGTGAGGGA14900.10810151966069183No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG14810.10744855746139906No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14800.10737600610592209No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG14720.10679559526210627No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14480.10505436273065889No Hit
CTATTATTGTCAGTCTTATGACAGCAGCAATCAAGGAGTGTTCGGCGGAG14440.104764157308751No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13930.1010640381794253No Hit
ACTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTG13920.10099148682394833No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA13850.10048362733560952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17950.056.945431
TCAACGC18200.056.1060754
CAACGCA19000.053.7515265
ATCAACG19250.052.292923
AACGCAG19950.050.8369646
TATCAAC20200.050.1895522
GGTATCA8400.047.9892231
ATACGCG605.142408E-447.95269
ACGCAGA22550.045.932427
CGCAGAG24000.042.557938
TATTGAG20750.033.274325
TTATTGA21450.032.5190124
GTACATG37500.031.6728861
TTTATTG25450.027.9700343
GATACGC1050.008012745527.4014848
GTAATAC23950.025.2469391
CAGAGTA27700.025.1880979
TGTATAC5150.025.1310633
ACATGGG45900.025.0641443
GTATATA6400.024.7444441
Produced by FastQC (version 0.11.5)