FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107920
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC16980.15326016318867788No Hit
TACTAGTACTGATCATCTTTATGTCTTCGGAAATGGGACCAAGGTCACCG15670.14143620478012853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12540.11318506751389992No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20450.072.977641
TCAACGC24650.060.5378344
ATCAACG25500.058.5199133
CAACGCA25700.057.5034945
TATCAAC26750.055.7853362
AACGCAG26650.055.1756676
GGTATCA10200.055.1324351
ACGCAGA29750.049.4017147
CGCAGAG29850.049.2051058
GCAGAGT38250.038.5857669
GTACATG31200.027.2671761
TACATGG32000.026.8083742
TTTCGCG1400.001041099925.7126439
CAGAGTA36950.023.965999
ACATGGG35550.022.9146043
GTACTAG12600.022.8877011
GTATTAT6650.020.5989321
ATGGGAG16350.019.8412155
TATACCA6950.019.7079895
CTAGTAC11500.019.4328863
Produced by FastQC (version 0.11.5)