FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107920
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC15300.13809661347389704No Hit
TACTAGTACTGATCATCTTTATGTCTTCGGAAATGGGACCAAGGTCACCG14750.13313235612679614No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15200.062.186141
TCAACGC15450.060.213124
ATCAACG15700.059.2596783
CAACGCA17100.054.4006235
AACGCAG18100.051.327696
TATCAAC19200.049.5907752
ACGCAGA19200.048.389237
CGCAGAG19400.047.890378
GCAGAGT26100.035.598289
GGTATCA7800.035.152391
GTACATG29850.031.1825221
TACATGG31150.029.1770062
GATACGT1000.00627320428.8097749
ACATGGG32550.027.6967143
CTAGTAC11600.024.2479953
GTACTAG13150.023.0456751
TACACCG2303.2751326E-521.9472685
GTATAGA5300.021.7826141
CATGGGG21750.021.2202574
ATATACT10050.020.809514
Produced by FastQC (version 0.11.5)