FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257837
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC57580.2550228382296862No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49850.22078653153438446No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTG40310.1785337028315153No Hit
GTCCTGGGCCCACTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC34090.1509852128386593No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33080.146511904978083No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30970.13716667766539392No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTT30720.1360594232444592No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAG30610.13557223129924792No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA28140.12463255762041282No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27320.1210007631197469No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25100.11116834386184654No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT24920.11037112067877354No Hit
GGTTATAACTATGTCTCCTGGTACCAACAAACCCCAGGCAAAGCCCCCAA24410.1081123216600667No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24340.10780229042220497No Hit
CTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATAACTATGTCTCCT24230.10731509847699369No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC23570.10439194680572601No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTTCTGCAGCTCATAT23270.10306324150060435No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA22890.10138021478078355No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53700.081.161471
GGTATCA22600.074.270491
ATCAACG65700.064.991433
TCAACGC66300.064.403274
CAACGCA68700.062.256815
TATCAAC69500.061.6437842
AACGCAG72600.058.811956
ACGCAGA84150.050.6530387
CGCAGAG84800.050.3496868
GCAGAGT105050.040.7810869
GTACATG69250.029.4398421
TACATGG69900.028.5338082
ACATGGG72600.026.9773353
CAGAGTA93700.026.8178379
GTATATA12750.025.9906061
CATGGGG46100.024.2101544
CCTAGGT39800.023.3380092
CTAGGTC42750.022.4017663
TAGGTCA43350.021.9256064
TATTGAG30950.021.170835
Produced by FastQC (version 0.11.5)