FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5607995
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC260790.46503251161957176No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCAACGACTGCTA198160.35335266882370614No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTCACCATCTCCTGCA188810.33668004340232116No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCA168810.3010166735170056No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC165110.2944189500882223No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTTT155620.2774966810776401No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT145800.2599859664639501No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTCACCATCTCCTGCACCGGCAGCAG141400.25214002508918076No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT132210.23575270662687825No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA128920.22988608228074384No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA127550.22744314144359973No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC126100.22485754712691436No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA126060.22478622038714374No Hit
GTCTATTACTGTGCAAGATACGTAGTGAGCGCGGCCTACTTTGACTATTG125010.22291389346816465No Hit
CTCCTGCACCGGCAGCAGTGGCAACATTGCCAACAACTTTGTGCAGTGGT121060.21587037791581482No Hit
GTAATAGACAGCCGTGTCCTCGTCTCTCAGACTGTCCATTTGCAGATACA119570.21321345685935883No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT119430.21296381327016162No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGG111800.19935823765891372No Hit
CTTCATGCGTGACCTGGCAGATGTAGCTTCTGTGGGACTTCCACTGCTCA108800.19400873217611642No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCAACGACTGCTAA107270.1912804843798898No Hit
CTATGAGGATGACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCT104960.18716136515813583No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC104480.18630544428088827No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG102500.18277477066224204No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGAGGAT101700.1813482358668294No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGATGTAGCTTCT98710.17601656206897473No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC98200.1751071461368992No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG97970.17469701738321808No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG97660.17414423514999566No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGATGTAGCTTCTGTGGGACTTCCA96660.1723610666557299No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA96030.17123767050434247No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGAGG94750.16895521483168227No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG92590.16510357088406818No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91200.16262496667703877No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA91020.1623039963480709No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGT89410.15943309507230302No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA88570.15793523353711977No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT87120.1553496392204344No Hit
GACTACTACTGTCAGTCTTTTGATATTAGCAGTCGTTGGATATTCGGCGG86840.15485035204203998No Hit
ACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGC86640.15449371834318684No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACA86000.15335249050685673No Hit
CCCTTCATGCGTGACCTGGCAGATGTAGCTTCTGTGGGACTTCCACTGCT85700.152817539958577No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG85050.15165848043730423No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG83970.1497326584634972No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGG81460.14525690554289009No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA80720.14393736085713343No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC78350.13971125152572353No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT77250.1377497661820312No Hit
TCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGC76960.1372326473186941No Hit
CTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCT73790.1315800031918716No Hit
GATCTATGAGGATGACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTG71660.1277818542990855No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC69860.1245721510094071No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTT69510.12394804203641409No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG68360.12189739826800844No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG67590.12052435852742378No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGA67570.12048869515753848No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC67250.11991808123937343No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGATGTAGCTTCTGTGG66830.11916915047178181No Hit
CTACTACTGTCAGTCTTTTGATATTAGCAGTCGTTGGATATTCGGCGGAG65810.11735031860763072No Hit
GTCTTCAGTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGAT64930.11578113033267684No Hit
TCCTCATAGATCACAGTGGTGGGGGCACTGCCCGGGCGCTGCTGGTACCA64640.11526401146933975No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGATGTAGCTTCTGT62590.11160851605609493No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT61720.11005715946608369No Hit
TCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCC60350.10761421862893958No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG59490.10608069372387101No Hit
TCCAACGACTGCTAATATCAAAAGACTGACAGTAGTAGTCAGCCTCGTCC58930.10508211936708217No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA57400.10235387157085554No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41300.083.024971
GTATCAA122450.076.7721251
TCAACGC140950.065.746944
ATCAACG141250.065.709253
TATCAAC146450.063.5716482
CAACGCA150050.061.7568665
AACGCAG156800.059.096226
CGCAGAG173050.053.546888
ACGCAGA186900.049.617377
GTACATG161900.035.7734681
TACATGG164500.034.8856432
CAGCCGT54100.034.202919
ACATGGG166400.034.185023
TATTGAG98000.034.164075
TAGACAG61950.031.4971075
ATAGACA66300.031.3867514
GTAATAG60700.031.330481
TTATTGA107100.031.3298634
CATGGGG124500.030.594854
AATAGAC62750.029.7199523
Produced by FastQC (version 0.11.5)