FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980747
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACGGCCGTGTCCTCGCCTCTCAGGCTGCTCATTTGCAGATACA27990.2853947042407471No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16580.16905481230123567No Hit
GTTTATTACTGTGCGAAACTCACCCCCCTTTTGACGGCCTTTGACTTCTG15330.15630942536658282No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14850.1514151967836761No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14330.14611311581886052No Hit
GTATTATTGCAGTTCATATGCAGGCAGCAACAATTTTTATGTCTTCGGAA14000.14274833366811215No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12380.12623031220080205No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12380.12623031220080205No Hit
GTATGTGCTACCACCACTACCAGTAATAGTTGAGACCCACTCCAGCCCCT11880.12113215742694089No Hit
GCAATAATACTCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGG11040.11256725740685417No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11000.11215940502494527No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10980.11195547883399082No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT10960.11175155264303638No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT10860.11073192168826415No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA10660.1086926597787197No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10600.10808088120585635No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA10520.10726517644203858No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10420.10624554548726634No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT10280.10481806215058521No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13100.062.808141
ATCAACG13100.058.9062923
TCAACGC13050.058.579354
CAACGCA14000.054.598745
AACGCAG15000.051.3686686
TATCAAC15350.051.2114752
CGCAGAG16600.045.9836658
GGTATCA5900.042.8152431
GCAGAGT20500.037.938119
TACATGG25100.032.7552032
GTACATG25500.031.700071
GTACACG9200.031.3528445
TAAACGG13150.031.2574755
ACATGGG25950.030.570643
TACACGG9700.029.6957326
ATAAACG14400.029.5487234
AACGGCC13750.029.3285857
TCTACAC5750.028.8475653
ACACGGG10000.028.804867
GTATGTA12350.028.0515371
Produced by FastQC (version 0.11.5)