FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3235592
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTC173380.5358524807824967No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC147800.45679430533886844No Hit
ATCATAGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC123100.3804558794804784No Hit
CCCTATGATTACCACTGCTGTCTGCTGAGTAGCAATAGTAGTCAGCGTCG116700.36067588249692795No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCTATGATTACCA98200.3034993287163524No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC87880.27160408358037724No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA76360.23600008900998642No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT75950.23473293295322772No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA75410.23306399570774067No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCT70670.21841443544179862No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATCAGT68830.21272768630902786No Hit
GTAATACATGGCCGTGTCCTCAGGTCTCAGCCTGGTCAACTCCATGTAGA63260.1955129076842816No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACG63100.1950184077596928No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG62710.19381306419350772No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC61730.19078425215540157No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA58450.18064700370133194No Hit
TCTATGAGGACGCTAAACGACCCGCCGGGACCCCTGAGAGATTCTCTGGC57680.17826722281424853No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA57460.17758728541793897No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGT56410.17434212966282522No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC56350.17415669219110444No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA56190.1736621922665157No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG55400.17122059888885868No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG54530.16853175554890726No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG52420.162010537793393No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT52270.16154694411409104No Hit
GGTGTGTCTCATCAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA49850.154067632754686No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCTATGATTACCAC49370.15258413298091972No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT49240.15218235179219136No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG48990.1514096956600214No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCC48800.15082247699957227No Hit
CCCCCATGCTGGTCATCTATGAGGACGCTAAACGACCCGCCGGGACCCCT46420.14346679062131443No Hit
GTCAGGGACAATGGCCACCTTGACCATCAGTGGGGCCCAGGTGGAGGACG46200.1427868532250049No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCG46160.14266322824385771No Hit
ATGTATTACTGTGCGAGAGATGTGATTGGGAGTAGCAGTTGGTTCGTGGG46050.1423232595457029No Hit
GTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG45740.1413651659418122No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45330.1400980098850535No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT44780.13839816639427963No Hit
GCTCTGGAGATGCGTTCCCAAAAAAATATGCTTATTGGTACCAGCAGAAG44750.13830544765841923No Hit
GACTACTATTGCTACTCAGCAGACAGCAGTGGTAATCATAGGGTGTTCGG44200.13660560416764536No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA43510.13447307324285632No Hit
GGGCCACACTGGTGTGTCTCATCAGTGACTTCTACCCGGGAGCCGTGACA43340.13394766707298078No Hit
ATAGTAGTCAGCGTCGTCCTCCACCTGGGCCCCACTGATGGTCAAGGTGG41160.1272101056004589No Hit
CTACTATTGCTACTCAGCAGACAGCAGTGGTAATCATAGGGTGTTCGGCG40520.12523210590210385No Hit
ACACTGGTGTGTCTCATCAGTGACTTCTACCCGGGAGCCGTGACAGTGGC39770.12291413750559403No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC39740.12282141876973364No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC39020.1205961691090842No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTGATGAGACACAC38550.11914357558060473No Hit
GTCCCTGACATGGAGCCAGAGAATCTCTCAGGGGTCCCGGCGGGTCGTTT38250.1182163882220008No Hit
ATGATTACCACTGCTGTCTGCTGAGTAGCAATAGTAGTCAGCGTCGTCCT35780.1105825456361618No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG35460.10959354578698427No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT35350.1092535770888295No Hit
GTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCC35330.10919176459825591No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCGTTCCCAAAA35020.10823367099436518No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG34880.10780098356035No Hit
GTCTGGGGCTGAGGTGAAGAAGCCAGGGGCCTCAGTGAAGCTCTCCTGTA34220.10576117137142135No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC33620.1039067966542135No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT33330.10301051554089638No Hit
GATTACCACTGCTGTCTGCTGAGTAGCAATAGTAGTCAGCGTCGTCCTCC33190.10257782810688121No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22450.078.8959661
GTATCAA61000.075.18751
ATCAACG71450.062.578543
TCAACGC72350.061.999124
TATCAAC73750.060.4316832
CAACGCA84400.052.8061185
AACGCAG86250.051.589996
CGCAGAG88000.050.482238
ACGCAGA97200.045.7040757
GCAGAGT100000.044.6362239
GAATACG8200.040.386839
GTACATG83050.036.672181
TATTGAG64450.035.1903575
TTATTGA65750.034.494584
ACATGGG86900.034.3017733
GTAATAC35950.031.8444881
GTGCTAG12200.031.2789421
ATGGCCG36800.030.1306828
ATTGAGT46400.029.7933466
CAGAGTA98400.029.7048059
Produced by FastQC (version 0.11.5)