FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862090
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC47260.25380083669425213No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34880.1873164025369343No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTG30920.1660499761021218No Hit
GTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTAGCAGATACA30030.16127040046399477No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28770.15450381023473625No Hit
GTCCTGGGCCCACTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC27250.14634093948197993No Hit
GTGTATTACTGTGCGAAAGATCTCCGATACAGTTATGGTTACGGGGCTAT26320.14134655145562244No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAG25980.13952064615566379No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25480.13683549130278344No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTT24050.12915594842354558No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23500.12620227808537718No Hit
GGTTATAACTATGTCTCCTGGTACCAACAAACCCCAGGCAAAGCCCCCAA22430.1204560467002132No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT21580.11589128345031657No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA21530.11562276796502854No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20910.11229317594745689No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC19820.1064395383681777No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19230.10327105564177887No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTTCTGCAGCTCATAT19050.10230439989474194No Hit
CTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATAACTATGTCTCCT18740.10063960388595611No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37750.084.148241
GGTATCA15500.081.3260651
TCAACGC50150.062.3113634
ATCAACG50250.062.187363
TATCAAC53850.058.2974052
CAACGCA55000.057.0769275
AACGCAG57200.054.8801886
CGCAGAG62400.050.0760738
ACGCAGA65350.047.7053877
GCAGAGT78750.040.136469
GTACATG53550.029.3238121
CAGAGTA67900.028.099679
TACATGG54100.028.0823632
ACATGGG55200.027.2618713
TAGCGCG2551.0149597E-725.41274
CATGGGA21350.023.2701764
AGTACGG2852.97312E-722.7370685
CTGTGCG19800.022.5450739
TATTGAG26450.022.321585
GTATATA11400.022.1149831
Produced by FastQC (version 0.11.5)