FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855381
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAATAATCAGCCTCATCGTCGGACCGGAGCCCCGTGATGGCCAGGGAGG29580.345810814128441No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA26210.30641316559521425No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25290.29565772445261235No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC24810.2900461899434287No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT24260.28361630665165577No Hit
GCCTTGGGCTGACTGAGGACGGTCAGTTTCGTCCCCCCGCCGAACACCGG23550.2753159118568217No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23420.27379612126058445No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23370.27321158641587784No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC21100.24667370446619696No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20190.2360351702925363No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT20120.23521682150994702No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG19860.23217724031747256No Hit
TCCTCAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC19710.2304236357833527No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19190.22434447339840374No Hit
GATTATTTCTGTGCAGCATGGGATGACAGCCTGAATGGTCCGGTGTTCGG18870.22060345039228133No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG18520.21651170647933493No Hit
ATCCCATGCTGCACAGAAATAATCAGCCTCATCGTCGGACCGGAGCCCCG18480.2160440786035696No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC18250.21335521831791915No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17920.2094972883428554No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT17250.20166452142378657No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG17210.2011968935480213No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16620.19429938238048308No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT15780.18447919698941173No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA15730.18389466214470512No Hit
GCATGGGATGACAGCCTGAATGGTCCGGTGTTCGGCGGGGGGACGAAACT15650.1829594063931745No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCTC15630.18272559245529185No Hit
GTAATAGACGGCCGTGTCGGCGGCAGTCACAGAGCTCAGCCTCAGGGAAA15620.18260868548635054No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA15450.180621267014348No Hit
GACTGAGGACGGTCAGTTTCGTCCCCCCGCCGAACACCGGACCATTCAGG15290.17875075551128677No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA14920.17442519766045775No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14730.17220396525057255No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14640.17115180253010062No Hit
GGGTATACTGGTACCAGCAACTCCCAGGAGCGGCCCCCAAATTCCTCATG14410.16846294224445013No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA13700.16016254744961603No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT13630.15934419866702673No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13140.1536157571889018No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12680.14823803661760082No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG12300.14379557179783045No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC12120.14169124635688657No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT11510.1345599212514657No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG11490.13432610731358305No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11160.13046817733851934No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC11080.12953292158698873No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG11070.1294160146180474No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11030.1289483867422821No Hit
TTCCTAAACATGAGGAATTTGGGGGCCGCTCCTGGGAGTTGCTGGTACCA10980.12836385189757546No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT10590.12380448010886377No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA10580.12368757313992246No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10560.1234537592020398No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC10550.12333685223309848No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10230.11959582922697605No Hit
GTCTATTACTGTGCGAGAGGCGAAGACTACTATGATTCTGGTGGTCACTT10170.1188943874133281No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG10160.11877748044438678No Hit
GGAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTTTCTGGCTCCAAG10080.11784222469285617No Hit
GACTATTCCTAAACATGAGGAATTTGGGGGCCGCTCCTGGGAGTTGCTGG10040.11737459681709086No Hit
TTTCTGTGCAGCATGGGATGACAGCCTGAATGGTCCGGTGTTCGGCGGGG9740.11386738774885109No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC9690.11328285290414447No Hit
CCCCTGAGGGCCGCTGACTATTCCTAAACATGAGGAATTTGGGGGCCGCT9610.11234759715261387No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9610.11234759715261387No Hit
GTTTAGGAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTTTCTGGCT9350.10930801596013939No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9300.10872348111543279No Hit
ATTTATTACAGTGGGAAAACCAACTACAACCCCTCCCTCAACGGTCGAGT9260.10825585323966747No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9210.10767131839496083No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA9180.10732059748813685No Hit
GTAATTGGGTATACTGGTACCAGCAACTCCCAGGAGCGGCCCCCAAATTC9120.1066191556744889No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9110.10650224870554759No Hit
TCCCAGGAGCGGCCCCCAAATTCCTCATGTTTAGGAATAGTCAGCGGCCC9100.10638534173660626No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC9100.10638534173660626No Hit
TCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCA9090.10626843476766494No Hit
TCCTAAACATGAGGAATTTGGGGGCCGCTCCTGGGAGTTGCTGGTACCAG8980.10498245810931035No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG8950.10463173720248638No Hit
TCCCTGGCCATCACGGGGCTCCGGTCCGACGATGAGGCTGATTATTTCTG8880.10381338841989708No Hit
GCACAGAAATAATCAGCCTCATCGTCGGACCGGAGCCCCGTGATGGCCAG8850.10346266751307312No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8830.10322885357519047No Hit
GTCCGGTGTTCGGCGGGGGGACGAAACTGACCGTCCTCAGTCAGCCCAAG8680.10147524904107058No Hit
CTCCTGGGAGTTGCTGGTACCAGTATACCCAATTACTTCCGATGTTGGAG8620.10077380722742263No Hit
ACACCGGACCATTCAGGCTGTCATCCCATGCTGCACAGAAATAATCAGCC8580.10030617935165734No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6400.074.320961
GTATCAA20150.065.094411
ATCAACG21000.062.105553
TATCAAC21200.061.5196462
TCAACGC21200.061.5196464
CAACGCA21700.059.4380345
AACGCAG22200.058.4170846
ACGCAGA24950.051.382757
CGCAGAG25650.049.9629678
GCAGAGT28650.044.4799429
TAGACGG6300.043.462455
ATAGACG6600.041.486884
AGACGGC6600.040.3903966
TACATGG23600.037.8600162
GTACATG24750.037.8543241
GTGCTAG4050.037.369011
GACGGCC7200.037.011547
AATAGAC7400.037.0018123
GTAATAG7700.036.5023841
GAATACG4150.034.6974459
Produced by FastQC (version 0.11.5)