FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855381
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAATAATCAGCCTCATCGTCGGACCGGAGCCCCGTGATGGCCAGGGAGG27170.31763623461358154No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA26490.30968656072557144No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC25340.29624225929731896No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25080.2932026781048445No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT23740.2775371442667069No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23570.2755497257947043No Hit
GCCTTGGGCTGACTGAGGACGGTCAGTTTCGTCCCCCCGCCGAACACCGG23360.2730946794469365No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21760.2543895644163244No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC19970.23346321697582714No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19480.22773477549770219No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG18980.22188942705063588No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT18690.21849912495133747No Hit
TCCTCAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC18200.2127706834732125No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT18040.21090017197015135No Hit
GATTATTTCTGTGCAGCATGGGATGACAGCCTGAATGGTCCGGTGTTCGG17890.20914656743603144No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA17730.20727605593297022No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG17530.2049379165541437No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17370.20306740505108248No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG16690.19511773116307235No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC16680.19500082419413103No Hit
ATCCCATGCTGCACAGAAATAATCAGCCTCATCGTCGGACCGGAGCCCCG16540.19336412662895247No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA15820.18494682486517702No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA15320.17910147641811075No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15240.17816622066658014No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCTC15230.17804931369763882No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT15090.1764126161324603No Hit
GCATGGGATGACAGCCTGAATGGTCCGGTGTTCGGCGGGGGGACGAAACT15040.17582808128775365No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA14760.17255468615739653No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA14660.17138561646798325No Hit
GTAATAGACGGCCGTGTCGGCGGCAGTCACAGAGCTCAGCCTCAGGGAAA14310.1672938725550369No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14050.1642542913625624No Hit
GACTGAGGACGGTCAGTTTCGTCCCCCCGCCGAACACCGGACCATTCAGG13810.16144852410797061No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13410.15677224535031756No Hit
GGGTATACTGGTACCAGCAACTCCCAGGAGCGGCCCCCAAATTCCTCATG13400.15665533838137624No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT12810.14975782721383804No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA12590.1471858738971289No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12240.14309412998418247No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG11750.13736568850605754No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT11530.13479373518934837No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC11480.13420920034464173No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11390.1331570376241698No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11350.1326894097484045No Hit
TTCCTAAACATGAGGAATTTGGGGGCCGCTCCTGGGAGTTGCTGGTACCA11150.130351270369578No Hit
GTCTATTACTGTGCGAGAGGCGAAGACTACTATGATTCTGGTGGTCACTT11020.12883147977334075No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT11000.12859766583545812No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG10490.1226354104194505No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA10330.12076489891638931No Hit
ATTTATTACAGTGGGAAAACCAACTACAACCCCTCCCTCAACGGTCGAGT10330.12076489891638931No Hit
GTTTAGGAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTTTCTGGCT9990.11679006197238423No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9950.11632243409661894No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG9950.11632243409661894No Hit
CCCCTGAGGGCCGCTGACTATTCCTAAACATGAGGAATTTGGGGGCCGCT9850.11515336440720567No Hit
GACTATTCCTAAACATGAGGAATTTGGGGGCCGCTCCTGGGAGTTGCTGG9760.11410120168673374No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC9750.11398429471779242No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA9750.11398429471779242No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9710.11351666684202712No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9460.11059399261849398No Hit
GGAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTTTCTGGCTCCAAG9450.11047708564955265No Hit
TTTCTGTGCAGCATGGGATGACAGCCTGAATGGTCCGGTGTTCGGCGGGG9370.10954182989802205No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG9130.10673606264343023No Hit
GTCATCCCATGCTGCACAGAAATAATCAGCCTCATCGTCGGACCGGAGCC8930.10439792326460372No Hit
CTCCTGGGAGTTGCTGGTACCAGTATACCCAATTACTTCCGATGTTGGAG8930.10439792326460372No Hit
GCACAGAAATAATCAGCCTCATCGTCGGACCGGAGCCCCGTGATGGCCAG8870.10369648145095577No Hit
TCCTAAACATGAGGAATTTGGGGGCCGCTCCTGGGAGTTGCTGGTACCAG8800.1028781326683665No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC8720.1019428769168359No Hit
GTAATTGGGTATACTGGTACCAGCAACTCCCAGGAGCGGCCCCCAAATTC8680.10147524904107058No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC8620.10077380722742263No Hit
GTCCGGTGTTCGGCGGGGGGACGAAACTGACCGTCCTCAGTCAGCCCAAG8620.10077380722742263No Hit
GGTACCAGCAACTCCCAGGAGCGGCCCCCAAATTCCTCATGTTTAGGAAT8610.10065690025848131No Hit
TGTTTAGGAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTTTCTGGC8560.10007236541377468No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG8560.10007236541377468No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4200.067.013471
ATCAACG13200.062.8444483
TCAACGC13200.062.8444484
GTATCAA14500.059.2278251
TATCAAC14600.057.3157042
CAACGCA14850.055.8715635
AACGCAG15200.054.495556
ACGCAGA15950.051.930027
CGCAGAG16600.049.4627348
GCAGAGT19400.042.3237849
GTACATG24350.042.0858571
TACATGG24150.041.8195572
GTGCTAG3500.041.2390631
CCATACG700.001083152841.2197154
ATAGACG5550.038.9916234
TAGACGG5400.038.7457125
TATTGAG21650.038.3229875
ATTGAGG10200.036.7206156
GAATACG3750.036.4925049
TTATTGA23100.035.5988464
Produced by FastQC (version 0.11.5)