FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327768
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATAATCAGCGGCCCTCTGGGGTCCCCGACCGATTCTCTGGCTCCAAG113050.48565836457928796No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC108640.4667131776018916No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCGGACCATTCAGG90680.38955772224723423No Hit
GTCTGGGATGACAACCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGGT89490.3844455289358733No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC86180.3702258988009114No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT81450.3499060043784432No Hit
GATTATTAGTATGTATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGG76450.32842620054919563No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCGG72650.31210154963896747No Hit
ATCCCAGACTGCACAGAAATAATCAGCCTCATCCTCAGACTGGAGCCCAC69050.2966360908819092No Hit
CCTCATACATACTAATAATCAGCGGCCCTCTGGGGTCCCCGACCGATTCT68760.29539026225981285No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC65140.2798388842874376No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC63140.2712469627557385No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT58340.25062635107966086No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA58080.24950940128053997No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT56980.24478384443810552No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC54300.23327066958562878No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG50660.21763337239793656No Hit
TACTAATAATCAGCGGCCCTCTGGGGTCCCCGACCGATTCTCTGGCTCCA50630.21750449357496107No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC50520.21703193789071765No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA45900.19718459915249284No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCGGACCATTCAGGT45820.1968409222912249No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA45720.19641132621463997No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT45580.19580989170742102No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC43460.18670245488382003No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA43380.18635877802255207No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG42320.18180505961075158No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC41880.1799148368737778No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41550.17849716982104746No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA41370.17772389688319454No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA40440.1737286533709545No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT38680.16616776242305936No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC38120.1637620243941836No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA37770.16225843812613627No Hit
GAAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG36580.15714624481477535No Hit
GATTATTTCTGTGCAGTCTGGGATGACAACCTGAATGGTCCGGTGTTCGG36160.15534194129311857No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT36100.15508418364716758No Hit
GTCTGAGGATGAGGCTGATTATTTCTGTGCAGTCTGGGATGACAACCTGA35630.1530650820872183No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG34360.14760921191458942No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC33840.14537531231634768No Hit
TTATTAGTATGTATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGGTA33710.14481683741678725No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGG33620.14443020094786077No Hit
GTTATACTGTGAACTGGTACCAGCAGTTCCCAGGAACGGCCCCCAAACTC33540.14408652408659284No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA33530.14404356447893432No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT33260.14288365507215495No Hit
CTCCTCATACATACTAATAATCAGCGGCCCTCTGGGGTCCCCGACCGATT32250.13854473469864695No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG32000.13747074450718458No Hit
CCTCTGGGGTCCCCGACCGATTCTCTGGCTCCAAGTCTGGCACTTCAGCC31770.13648267353103918No Hit
TGTATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCAGTTCAC31700.1361819562774297No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG30960.13300294531070106No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG30940.13291702609538408No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30330.13029649002821586No Hit
GTAATAAAGACCCGTGTCCCCGGCTCTCAGGGTGTTCATTTGAAGATACA28920.12423918534836804No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC28870.12402438731007558No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCC28580.12277855868797921No Hit
GTATAACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT27580.1184825979221297No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT27380.11762340576895979No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG27290.11723676930003335No Hit
TTTCTGTGCAGTCTGGGATGACAACCTGAATGGTCCGGTGTTCGGCGGAG27270.11715085008471633No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAAGTGCCAGACTT26970.11586206185496149No Hit
CCCCAGAGGGCCGCTGATTATTAGTATGTATGAGGAGTTTGGGGGCCGTT26880.11547542538603503No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG26740.1148739908788161No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG26310.1130267277495008No Hit
AAGTTATACTGTGAACTGGTACCAGCAGTTCCCAGGAACGGCCCCCAAAC26290.11294080853418381No Hit
TCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCA26030.11182385873506294No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT25800.11083578775891754No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25620.11006251482106465No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA25520.1096329187444797No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG25150.10804341326111537No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25130.10795749404579838No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG24820.10662574620838502No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG24740.10628206934711706No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGT24060.1033608160263394No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG23550.10116987603575613No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT23500.10095507799746367No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)