FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650510
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC19690.302685585156262No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAACTGGTGGAGTCTGGGGGAGGCG16070.24703694024688322No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15650.2405804676330879No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14950.22981967994342903No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGTTCCCTACTGTTT13970.21475457717790658No Hit
GTGTATCTGCAGATGAACAGGCTGAAAGCTGAGGACACGGCTGTGTATTA13050.20061182764292632No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12980.19953574887396044No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG12810.19692241472075755No Hit
GTAATACACAGCCGTGTCCTCAGCTTTCAGCCTGTTCATCTGCAGATACA12420.19092711872223334No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12360.19000476549169112No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12320.18938986333799634No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT12020.1847780971852854No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11890.18277966518577732No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA11890.18277966518577732No Hit
GTGTTGGAAGTATTCAACGAAAGCGTAGTCAGCCCGACAGTAATACACAG11800.18139613533996402No Hit
GATTATTACTGCTGCTCATATGCAAACAGTAGGGAACTGTTCGGCGGAGG11630.17878280118676115No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11380.17493966272616868No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGTTC11140.17125024980399994No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11040.16971299441976295No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10910.16771456242025487No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10880.16725338580498378No Hit
GTGTACTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10750.16525495380547572No Hit
CTTCCAACACTGGGGCCAGGGCACCCTGGTCACCGTCTCTTCAGCCTCCA10580.16264161965227283No Hit
GTAGGGAACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC10430.16033573657591735No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10090.15510906826951162No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGT10070.15480161719266422No Hit
GTTTGGAGGGTGTACTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10040.1543404405773931No Hit
CTCCAGAGACAATGCCAAGAACACGGTGTATCTGCAGATGAACAGGCTGA9940.15280318519315614No Hit
GTTCCCTACTGTTTGCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCA9860.15157338088576655No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTTTCCT9670.14865259565571629No Hit
GTGTATTACTGTCGGGCTGACTACGCTTTCGTTGAATACTTCCAACACTG9570.1471153402714793No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC9480.14573181042566602No Hit
ATACACAGCCGTGTCCTCAGCTTTCAGCCTGTTCATCTGCAGATACACCG9360.14388710396458163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9080.13958278888871808No Hit
GTATTACTGTCGGGCTGACTACGCTTTCGTTGAATACTTCCAACACTGGG9040.1389678867350233No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8900.1368157291970915No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGT8880.1365082781202441No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8750.13450984612073605No Hit
GCAGATACACCGTGTTCTTGGCATTGTCTCTGGAGATGGTGAATCGGCCC8430.1295906288911777No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8430.1295906288911777No Hit
CCAGTACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8220.12636239258428No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7970.12251925412368755No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGTTCCCTACTGTTTG7950.12221180304684018No Hit
GTACTTATGCTATCTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAG7890.12128944981629798No Hit
TCCCTACTGTTTGCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGC7890.12128944981629798No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA7850.12067454766260317No Hit
GGGCTGGAGTGGGTGGCAGTCATATCACATGTTGGAAATAATGAATACTA7810.1200596455089084No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA7690.11821493904782401No Hit
GTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG7580.11652395812516333No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG7570.11637023258673965No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA7540.11590905597146854No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC7510.11544787935619744No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG7500.11529415381777373No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT7490.11514042827935003No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA7470.11483297720250266No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTAGATAGCATAAGTAC7290.11206591751087608No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7250.11145101535718129No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT7230.1111435642803339No Hit
CTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCA7230.1111435642803339No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACTTATGCTATCTACTGGG7210.1108361132034865No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7060.10853023012713102No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7030.10806905351185991No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG6980.10730042581974145No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT6980.10730042581974145No Hit
AATGAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA6930.10653179812762294No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG6920.10637807258919924No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA6910.10622434705077555No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6910.10622434705077555No Hit
CAGTAGGGAACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGC6890.10591689597392814No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT6870.10560944489708077No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG6800.10453336612811487No Hit
GTCCAGTGTCAGGTGCAACTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC6740.10361101289757269No Hit
GCTGTGTATTACTGTCGGGCTGACTACGCTTTCGTTGAATACTTCCAACA6700.10299611074387788No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC6630.10192003197491198No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC6590.1013051298212172No Hit
CAGTAATACACAGCCGTGTCCTCAGCTTTCAGCCTGTTCATCTGCAGATA6550.1006902276675224No Hit
GGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA6540.1005365021290987No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13450.065.881321
ATCAACG13300.064.423013
TATCAAC13400.063.9422452
TCAACGC13500.063.458844
CAACGCA14100.060.7584655
AACGCAG14600.059.1707886
CGCAGAG16600.052.041778
GCAGAGT19350.045.76189
GGTATCA5650.044.6271441
TAGACTG1651.4624675E-939.2678875
GTTATAG750.001530019838.4218441
GTCTTAG951.06768464E-437.916291
CATGGGA6500.032.1191144
CTAGACA900.00375459531.9960524
GTACATG16000.031.9681761
TACATGG15400.031.7931772
TTATTGA8950.031.3704324
TATTGAG9000.031.1961545
ACATGGG15550.030.5604153
GTACACG5900.030.5047155
Produced by FastQC (version 0.11.5)