FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161349
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTACTATGCCACACCTGACAGTAATAGTCGGCCTCATCTCCGCCTTC65530.30319027607295257No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC64740.299535151426262No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTG55150.25516471425947407No Hit
TAGTAGTAGTGATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC52250.24174716808807833No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTA51520.2383696478449339No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA44990.20815703525899798No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43760.20246614498630253No Hit
CCTATGTGCTGACTCAGACACCCTCGGTGTCAGTGGCCCCAGGACAGACG43680.20209600578157438No Hit
CACTACTACTATGCCACACCTGACAGTAATAGTCGGCCTCATCTCCGCCT38770.17937871209138367No Hit
ATTCTATGCAGCCTCAGTGAAGGGCCGATTCACCGTCTCCAGAGACAACG38170.17660266805592248No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38040.17600119184823923No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA37490.17345648481573314No Hit
GTAGTAGTGATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA37200.17211473019859358No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT36270.16781186194362874No Hit
GCCTCTGTGACCTCCTATGTGCTGACTCAGACACCCTCGGTGTCAGTGGC35370.16364779589043693No Hit
CTACTATGCCACACCTGACAGTAATAGTCGGCCTCATCTCCGCCTTCGAC33420.15462565277518808No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32960.15249735234800116No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA32540.15055412152317835No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA32100.14851835589717347No Hit
CCCAATGATCACTACTACTATGCCACACCTGACAGTAATAGTCGGCCTCA31460.1455572422593482No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT31250.1445856268469368No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC31070.14375281363629844No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30970.14329013963038822No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30730.14217972201620377No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC30660.14185585021206662No Hit
CCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGCTGACTCAGACAC30250.13995888678783483No Hit
CCATAGTAGTACCAGGGGTAGCAACTACCGCCACTACAAGAGTCAACTCT30020.13889473657424137No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA29690.1373679123547377No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT29630.1370903079511916No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC29530.1366276339452814No Hit
CCCCTGTGCTGGTCGTCTATGGTGATAGCGACCGGCCCTCAGGGATTCCT29500.13648883174350834No Hit
GTACATGGGAGTCTGGACCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA29450.13625749474055324No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA29030.1343142639157304No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTAC28740.13297250929859084No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA27440.12695774722175826No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA27260.1261249340111199No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26970.12478317939398033No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC26740.12371902918038688No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG26340.12186833315674608No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26290.12163699615379099No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAAACAAGATTGGAGGT26150.12098925254551672No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25550.11821320851005553No Hit
GTAATAGTCGGCCTCATCTCCGCCTTCGACCCTGCTGATGGTCAGGGTGG25330.11719532569705308No Hit
TAGTAGTACCAGGGGTAGCAACTACCGCCACTACAAGAGTCAACTCTCGC24780.114650618664547No Hit
CTACTATGGTATGGACGTCTGGGGCCGAGGGACCACGGTCACCGTCTCTT23690.10960747200012586No Hit
GTGTACTTCTGTGCGAGAGTTGACTCTTGTAGTGGCGGTAGTTGCTACCC23690.10960747200012586No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT23610.10923733279539768No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT23570.10905226319303363No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG23330.10794184557884913No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT23260.107617973774712No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG23260.107617973774712No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGGTGAT23170.10720156716939283No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG22890.10590607995284428No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22690.10498073194102388No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22420.10373151212506634No Hit
GAAGTACACAGCCGAGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATATA21910.10137187469492431No Hit
GGATTACCTGTGGGGGAAACAAGATTGGAGGTGAAAGTGTGCACTGGTAC21820.10095546808960515No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)