FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161349
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTACTATGCCACACCTGACAGTAATAGTCGGCCTCATCTCCGCCTTC59590.27570744012188686No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC58840.2722373850775604No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTG47670.2205566986173913No Hit
TAGTAGTAGTGATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC46500.21514341274824195No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTA46100.21329271672460115No Hit
CCTATGTGCTGACTCAGACACCCTCGGTGTCAGTGGCCCCAGGACAGACG39630.18335770854221137No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA39340.18201595392507178No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39320.18192341912388976No Hit
GTAGTAGTGATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA34510.15966879943960924No Hit
ATTCTATGCAGCCTCAGTGAAGGGCCGATTCACCGTCTCCAGAGACAACG34410.15920612543369905No Hit
GCCTCTGTGACCTCCTATGTGCTGACTCAGACACCCTCGGTGTCAGTGGC33450.15476445497696115No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33010.1527286893509563No Hit
CACTACTACTATGCCACACCTGACAGTAATAGTCGGCCTCATCTCCGCCT32440.15009144751726816No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32290.14939743650840284No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT32190.14893476250249266No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT31580.14611245106644044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31260.14463189424752781No Hit
CTACTATGCCACACCTGACAGTAATAGTCGGCCTCATCTCCGCCTTCGAC29060.13445306611750346No Hit
CCATAGTAGTACCAGGGGTAGCAACTACCGCCACTACAAGAGTCAACTCT28240.13065913926903983No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA28230.1306128718684488No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA28100.13001139566076556No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT27860.1289009780465811No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27280.12621746881230195No Hit
CCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGCTGACTCAGACAC27060.1251995859992995No Hit
CCCCTGTGCTGGTCGTCTATGGTGATAGCGACCGGCCCTCAGGGATTCCT26910.12450557499043421No Hit
CCCAATGATCACTACTACTATGCCACACCTGACAGTAATAGTCGGCCTCA26890.12441304018925219No Hit
GTACATGGGAGTCTGGACCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA26780.12390409878275095No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26680.12344142477684077No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26260.12149819395201793No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC26110.12080418294315262No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA25950.12006390453369632No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA25810.11941616092542204No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA25780.11927735872364897No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA25310.11710279089587104No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTAC25110.11617744288405064No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24750.11451181646277395No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC24750.11451181646277395No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG24460.11317006184563437No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23570.10905226319303363No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23510.10877465878948749No Hit
TAGTAGTACCAGGGGTAGCAACTACCGCCACTACAAGAGTCAACTCTCGC23080.10678516056407364No Hit
CTACTATGGTATGGACGTCTGGGGCCGAGGGACCACGGTCACCGTCTCTT22770.10535087114575203No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAAACAAGATTGGAGGT22490.10405538392920348No Hit
GTAATAGTCGGCCTCATCTCCGCCTTCGACCCTGCTGATGGTCAGGGTGG22460.10391658172743042No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGGTGAT21830.10100173549019617No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36250.078.655531
GGTATCA15300.078.589981
ATCAACG46850.059.7863733
TCAACGC48000.058.3539924
TATCAAC49700.056.7926182
CAACGCA54350.051.9336365
AACGCAG56800.049.694696
CGCAGAG59100.047.638888
GCAGAGT65650.042.6605879
ACGCAGA69250.040.760417
GTACATG56550.037.9424821
TACATGG56900.037.5842932
ACATGGG57400.037.2569053
CATGGGA30250.034.7527924
CAGCGCT10850.031.8510289
ATGGGAG28650.031.667095
TATTGAG30550.030.1690035
AATCCGC6500.028.8026396
CAGAGTA64300.027.9924569
TTATTGA33200.027.977824
Produced by FastQC (version 0.11.5)