FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032502
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATGGCCGTGTCGTCAGATCTCAGGCTCCTCAGTTCCATGTAGA21630.2094911196297925No Hit
ATGTATTACTGTGCGAGAGATGGTTACTCTGGTTCGGGAAACCCTGACTA16280.15767523936999636No Hit
GTCTACATGGAACTGAGGAGCCTGAGATCTGACGACACGGCCATGTATTA15290.14808688021911823No Hit
GCATAATAGTTACCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCA11980.11602883093688923No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT11130.10779640136290294No Hit
CTATTATGCTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT11130.10779640136290294No Hit
CATGTAGACTGTGCTCGTGGATGGGTCTGTGGTCATGGTGACTCTGCCCT10810.10469713375857868No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12400.068.095251
TCAACGC14200.058.923724
ATCAACG14600.057.8034173
CAACGCA15550.054.264115
TATCAAC17100.049.7744452
AACGCAG17100.049.6995936
CGCAGAG19050.044.61448
ACGCAGA21950.038.7200137
GTAATAC14350.031.1811771
GCAGAGT31900.028.4504369
ATGGCCG14950.028.424898
AATCCGC1804.9481896E-628.0086696
TGGCCGT14700.027.929729
TAATACA18100.025.50482
AATACAT18100.025.50483
GTACATG28200.024.8242031
ATACATG17950.024.5124054
TACACGG5400.024.007436
ACATGGC19250.023.196796
AAATCCG2202.3212378E-522.9473535
Produced by FastQC (version 0.11.5)