FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480131
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACA86660.5854887168770873No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGACGGCCAGGGAGG77900.5263047662673102No Hit
GATTATTACTGTGCAGCGTGGGATGACAGCCTGACTGGTCCGGTGTTCGG63360.42807021810907275No Hit
TTCTATTACTGTGCAAAAGATATCTGGGGAGTTATTAGCCCTAACTGGTA61540.41577400919242957No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC61090.4127337377569958No Hit
GTAATACTCAGCGGCCCTCAGGTGTCCCTGACCGATTCTCTGGCTCCAAG49630.3353081585346162No Hit
GTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTA49060.3314571480497334No Hit
TAGTAATACTCAGCGGCCCTCAGGTGTCCCTGACCGATTCTCTGGCTCCA47960.3240253734297842No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCAGTCAGG46810.31625579087256467No Hit
GGTATAGGCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA43970.2970683000356049No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCACCCTCAGCGTCTGGGACCC42170.28490721429386995No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41700.281731819683528No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGG41140.27794837078609935No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCACCCTCAGCGTCT40350.2726110053772267No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT36240.24484319293359846No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT36060.24362708435942496No Hit
GCCTATACCACTATTCCAACTAATACCTGAGACCCACTCCAGGCCCTTCC34460.2328172303667716No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34070.23018232845606235No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33810.22842572718225615No Hit
GAGTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGG33660.22741230337044493No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA33270.22477740145973565No Hit
TCTATTACTGTGCAAAAGATATCTGGGGAGTTATTAGCCCTAACTGGTAC31920.2156565871534344No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA31910.2155890255659803No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC31880.21538634080361807No Hit
CTCCAGAGACAGCGCCAGGAACTCCCTGTATCTGCAAATGAACAGTCTGA31040.20971116745747506No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA30720.2075491966589444No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC29690.20059035315117377No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCAC29530.19950936775190845No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA29530.19950936775190845No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29280.19782032806555636No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA28680.19376663281831136No Hit
GTCTGAGAGCTGAGGACACGGCCTTCTATTACTGTGCAAAAGATATCTGG28340.1914695388448725No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC27050.18275409406329574No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG26630.17991650739022425No Hit
TTACTGTGCAGCGTGGGATGACAGCCTGACTGGTCCGGTGTTCGGCGGAG26490.17897064516586708No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA26210.17707892071715273No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26030.17586281214297925No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA26000.17566012738061698No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC25780.17417377245662716No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG25680.1734981565820863No Hit
GTCTCTGGAGATGGTGAATCGGCCCTTCACAGAGTCCGCATAGCCTATAC25580.1728225407075455No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG25510.1723496095953669No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCAGTCAGGC25400.17160643213337198No Hit
ATAGTAATACTCAGCGGCCCTCAGGTGTCCCTGACCGATTCTCTGGCTCC25150.1699173924470199No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT24410.1649178349754177No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24250.16383684957615238No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24200.16349904163888196No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATACTCAGCGGCCCT23800.16079657814071863No Hit
CATCTATAGTAATACTCAGCGGCCCTCAGGTGTCCCTGACCGATTCTCTG23630.1596480311539992No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23620.15958046956654512No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC23530.15897241527945838No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT23440.1583643609923716No Hit
TCCTCAGACTGGAGCCCACTGACGGCCAGGGAGGCTGACGTGCCAGACTT23210.15681044448092768No Hit
GCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGACGGCCAG22620.15282431082113676No Hit
GCCTTCTATTACTGTGCAAAAGATATCTGGGGAGTTATTAGCCCTAACTG22420.1514730790720551No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCA22350.1510001479598765No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG22250.1503245320853357No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC22150.14964891621079485No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22110.14937866986097853No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT21800.14728426064990194No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA21720.14674376795026928No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21240.14350081175247326No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG20820.14066322507940177No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG20730.140055170792315No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG19620.13255583458491174No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGT19500.13174509553546274No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG19460.13147484918564642No Hit
CTATTACTGTGCAAAAGATATCTGGGGAGTTATTAGCCCTAACTGGTACT19390.13100191807346784No Hit
TTATTACTGTGCAGCGTGGGATGACAGCCTGACTGGTCCGGTGTTCGGCG19280.1302587406114729No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGG19030.12856970092512082No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA18830.12721846917603916No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTG18740.12661041488895242No Hit
ACACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC18720.12647529171404423No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT18590.12559699107714115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18260.1233674586911564No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC18090.12221891170443697No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA17970.12140817265498796No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGAAAATTATGCCATGCACTGGG17970.12140817265498796No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA17870.12073255678044714No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGTT17800.12025962566826855No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCGTGGGATGACAGCCTGA17740.11985425614354406No Hit
ATAGAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACAGGG17670.11938132503136548No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA17580.11877327074427872No Hit
GCCCTCAGGTGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAG17580.11877327074427872No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT17540.11850302439446238No Hit
GTGTTGACGCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC17490.11816521645719197No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATGGCATAATTTT17430.11775984693246745No Hit
GGCCTGGAGTGGGTCTCAGGTATTAGTTGGAATAGTGGTATAGGCTATGC17270.11667886153320212No Hit
GATCAACACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGA17220.11634105359593172No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT17210.11627349200847763No Hit
GCTCTCAGACTGTTCATTTGCAGATACAGGGAGTTCCTGGCGCTGTCTCT17130.11573299930884497No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG17060.11526006819666638No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC16990.1147871370844878No Hit
TTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGGCT16680.1126927278734112No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC16630.1123549199361408No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC16580.11201711199887038No Hit
CAGTAATAGAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATA16570.11194955041141628No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG16560.1118819888239622No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG16550.11181442723650811No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT16510.1115441808866918No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT16390.11073344183724278No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16200.1094497716756152No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16190.10938221008816111No Hit
CTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGTATTAGTTGGAATAGTGGT16140.1090444021508907No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16070.1085714710387121No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG15900.1074229240519927No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT15810.10681486976490595No Hit
CAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGACGGCCAGGGA15780.10661218500254369No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC15700.10607169230291104No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG15640.10566632277818652No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG15630.10559876119073244No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC15580.10526095325346203No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC15430.10424752944165076No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCC15280.10323410562983952No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTGAAAATTATGCC15190.10262605134275277No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT15090.10195043546821192No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG15040.10161262753094152No Hit
CTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGCCTCCACCAAGGG14970.10113969641876294No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25700.066.7143251
GGTATCA11750.055.792881
TATCAAC30900.055.2243082
TCAACGC30950.055.138824
ATCAACG31200.054.6973
CAACGCA31650.054.146835
AACGCAG32400.052.893436
CGCAGAG34500.049.253078
TCCGTAC450.00990385748.004123
GCAGAGT38250.044.4243439
GAATACG1807.8216544E-1140.0007329
GTACATG36000.036.8205761
TACATGG36350.036.2482572
ACGCAGA47250.036.114957
ACATGGG36600.035.609613
GTAATAG39750.034.7968031
TAATAGA40200.034.388692
CATGGGG15100.033.857214
AGGCCGT40950.033.4072049
ATGGGAG19050.033.26275
Produced by FastQC (version 0.11.5)