FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480131
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACA79520.5372497434348716No Hit
GATTATTACTGTGCAGCGTGGGATGACAGCCTGACTGGTCCGGTGTTCGG56340.3806419837163062No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC52960.357806167156826No Hit
TTCTATTACTGTGCAAAAGATATCTGGGGAGTTATTAGCCCTAACTGGTA52920.35753592080700963No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCACCCTCAGCGTCTGGGACCC47660.32199852580616173No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGACGGCCAGGGAGG47100.3182150769087331No Hit
TAGTAATACTCAGCGGCCCTCAGGTGTCCCTGACCGATTCTCTGGCTCCA44820.3028110349692021No Hit
GTAATACTCAGCGGCCCTCAGGTGTCCCTGACCGATTCTCTGGCTCCAAG44590.30125711845775816No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCACCCTCAGCGTCT43540.29416315177507935No Hit
GTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTA41520.28051571110935447No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCAGTCAGG39790.2688275564797981No Hit
GGTATAGGCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA37360.25241009072845577No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35630.24072193609889933No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGG35160.23754654148855744No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT34220.23119575226787356No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCAC33210.22437203193501118No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT32900.2222776227239346No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32520.2197102824006794No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31530.21302168524272516No Hit
GAGTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGG31330.21167045349364347No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30260.20444136363605656No Hit
GCCTATACCACTATTCCAACTAATACCTGAGACCCACTCCAGGCCCTTCC29800.2013335306131687No Hit
TCTATTACTGTGCAAAAGATATCTGGGGAGTTATTAGCCCTAACTGGTAC28250.19086148455778576No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC28240.19079392297033168No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA28170.1903209918581531No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA26370.17815990611641808No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26130.17653842801752007No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26030.17586281214297925No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC25890.17491694991862206No Hit
CTCCAGAGACAGCGCCAGGAACTCCCTGTATCTGCAAATGAACAGTCTGA24920.16836347593557596No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24200.16349904163888196No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC24130.16302611052670338No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA23820.16093170131562679No Hit
GTCTGAGAGCTGAGGACACGGCCTTCTATTACTGTGCAAAAGATATCTGG23760.16052633179090228No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA23680.15998583909126962No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA23170.15654019813111136No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG23170.15654019813111136No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23090.1559997054314787No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23060.15579702066911644No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22870.15451335050748885No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22710.15343236510822353No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC22370.15113527113478467No Hit
TTACTGTGCAGCGTGGGATGACAGCCTGACTGGTCCGGTGTTCGGCGGAG22250.1503245320853357No Hit
GTCTCTGGAGATGGTGAATCGGCCCTTCACAGAGTCCGCATAGCCTATAC22250.1503245320853357No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATACTCAGCGGCCCT22240.15025697049788161No Hit
ATAGTAATACTCAGCGGCCCTCAGGTGTCCCTGACCGATTCTCTGGCTCC22230.15018940891042754No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCA21790.14721669906244786No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCAGTCAGGC21750.14694645271263151No Hit
GTGTTGACGCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC21660.14633839842554477No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21240.14350081175247326No Hit
CATCTATAGTAATACTCAGCGGCCCTCAGGTGTCCCTGACCGATTCTCTG21080.1424198263532079No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT20570.13897418539304968No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT20160.13620416030743224No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG19760.13350169680926893No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT19650.13275851934727398No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19630.13262339617236582No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG19490.13167753394800866No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA19480.13160997236055458No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG19400.1310694796609219No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC19300.13039386378638107No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19250.13005605584911065No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18720.12647529171404423No Hit
ACACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC18630.12586723742695746No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA18520.12512405996496256No Hit
GCCTTCTATTACTGTGCAAAAGATATCTGGGGAGTTATTAGCCCTAACTG18450.12465112885278397No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG18260.1233674586911564No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT18250.1232998971037023No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG17950.1212730494800798No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT17860.12066499519299305No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTG17810.12032718725572264No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGG17770.1200569409059063No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGAAAATTATGCCATGCACTGGG17530.1184354628070083No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA17480.11809765486973788No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC17030.11505738343430413No Hit
TTATTACTGTGCAGCGTGGGATGACAGCCTGACTGGTCCGGTGTTCGGCG16880.11404395962249289No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16780.11336834374795204No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCGTGGGATGACAGCCTGA16550.11181442723650811No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGTT16540.11174686564905405No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT16440.1110712497745132No Hit
CAGTAATAGAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATA16430.11100368818705911No Hit
CTATTACTGTGCAAAAGATATCTGGGGAGTTATTAGCCCTAACTGGTACT16350.11046319548742645No Hit
ATAGAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACAGGG16260.1098551412003397No Hit
TTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGGCT16260.1098551412003397No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC15910.10749048563944677No Hit
GCCCTCAGGTGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAG15870.10722023928963044No Hit
GGCCTGGAGTGGGTCTCAGGTATTAGTTGGAATAGTGGTATAGGCTATGC15770.1065446234150896No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGT15760.10647706182763553No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15670.10586900754054877No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG15660.10580144595309468No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA15540.1049907069036457No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATGGCATAATTTT15530.10492314531619161No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG15450.10438265261655895No Hit
GATCAACACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGA15330.10357191356710994No Hit
TCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCA15010.10140994276857927No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT14830.10019383419440576No Hit
CTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGTATTAGTTGGAATAGTGGT14810.1000587110194976No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9050.085.122821
GTATCAA24550.076.8352661
ATCAACG29650.062.1705553
TATCAAC29850.061.7540052
TCAACGC30200.061.2767454
CAACGCA32850.056.114375
AACGCAG33750.054.4046336
CGCAGAG36300.050.582828
GCAGAGT40050.046.1952559
ACGCAGA45450.040.3994837
ATGGGAG20550.036.0906645
GTACATG37950.035.2867741
TACATGG37850.035.1945462
ACATGGG38250.034.8264963
GTAATAG39400.034.5363461
TAATAGA39500.034.0889782
CATGGGA24100.033.4634324
AGCGCTG8500.032.190985
CAGCGCT8600.031.8166644
AGGCCGT40700.031.6610579
Produced by FastQC (version 0.11.5)