FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031311
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21530.20876340890381273No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGTCGAGAGACAGGGACA21210.20566056213886982No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT15780.15300913109624545No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14690.14244005930315878No Hit
GTCTCCAGGCACTCTGTCCTTGTCTCCAGGCGAGAGCGCCACCCTCTCGT14380.1394341764996204No Hit
GTCCAAGAACCAGTTGTCCCTGTCTCTCGACTCTGTGACCGCCGCGGACA14270.1383675729241713No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA14100.13671918558029536No Hit
GTACTACTGTCAACGGTGGTTTGAAACCTCACCCCGGTACACTTTTGGCC13920.13497383427501503No Hit
GTCTCCGGTGACTCCATCAGCAATGGTGCCTGGTGGAGTTGGGTCCGCCA13730.13313151900833017No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12380.12004138421872744No Hit
GTGTATTACTGTGCGAGACACCTTACCCAGTCTGGCACCAGGGGTTTTCA11690.11335087088181935No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11160.10821178092738273No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC11020.1068542854677202No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC10870.10539982604665324No Hit
TTCTTGGACTGGTCGACTGACATGGTGACTCGACTCTCGAGGGACGGGCT10580.10258787116592377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13150.043.2682044
GGTATCA7550.042.9355851
ATCAACG13500.042.146443
GTATCAA14550.041.092921
GAATACG2550.039.5263639
CAACGCA14850.038.3149455
TATCAAC15550.037.056922
AACGCAG15500.036.6957246
CGCAGAG17350.033.1978958
ACGCAGA17350.032.7829257
TACATGG26300.031.4957372
GTACATG27250.030.6650261
ACATGGG26250.030.4551833
GCAGAGT21450.026.8510749
TACACGG11600.026.6979545
TAATACG1658.8058776E-526.189964
ATACACG12100.026.189964
TCTACAC8000.024.3075563
CTAGAGA6650.023.8269584
CATGGGG14000.023.1500574
Produced by FastQC (version 0.11.5)