FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700558
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT32200.45963360635379225No Hit
GTTTTATACAGCTCCACCAATAACAACTACTTAGCTTGGTACCAGCAGAA29460.4205219268069168No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24850.3547172396860788No Hit
ATATTATGATAGTCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA22150.31617653356324527No Hit
ATAATACACAGCCGTGTCCTCGGCTCTAAGACTGCTCATCTGCAGATATA19330.2759229071682859No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17290.24680326254214496No Hit
TATTATGATAGTCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAA17230.245946802406082No Hit
TTATTACTGTCAGCAATATTATGATAGTCCCTGGACGTTCGGCCAAGGGA17130.24451936884597705No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16660.23781043111348382No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16570.23652574090938938No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16330.2330999003651375No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16140.23038777660093812No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15670.22367883886844486No Hit
ATCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGC15570.22225140530833992No Hit
GTATAAAACATTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA15340.21896830812009857No Hit
GTTTATTACTGTCAGCAATATTATGATAGTCCCTGGACGTTCGGCCAAGG14480.20669237950319605No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14400.20555043265511208No Hit
GTCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTG14030.20026892848272376No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13490.1925607872581571No Hit
GCTCTAAGACTGCTCATCTGCAGATATAGGGTGTTCTTGGCGTTGTCTCT13140.18756476979778974No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12920.18442441596555886No Hit
ATTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTGTTA12530.1788574250811496No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12440.17757273487705513No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12430.17742999152104466No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12340.1761453013169502No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12250.17486061111285575No Hit
GATATAGGGTGTTCTTGGCGTTGTCTCTGGAGATGATAAATCGGCCCTTC12170.17371866426477178No Hit
TATTAACACTGATGGGACTACCAAAAGTTACGCGGACTCCGTGAAGGGCC11880.16957910694046746No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11740.16758069995632055No Hit
CTCCTAAATTGCTCATTTATTGGGCATCTACCCGGGACTCCGGGGTCCCT11640.16615326639621558No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11580.16529680626015264No Hit
TCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG11150.15915884195170135No Hit
ACCCGGGACTCCGGGGTCCCTGACCGATTCACTGGCAGCGGGTCTGGGAC11140.15901609859569085No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11080.15815963845962788No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11020.1573031783235649No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG10830.15459105455936553No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10650.15202167415117662No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10630.15173618743915565No Hit
GTCCCTGACCGATTCACTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC10470.14945229374298774No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10440.14902406367495624No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC10410.14859583360692477No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA10400.14845309025091427No Hit
TCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT10230.14602645319873586No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9720.13874654204220066No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9680.13817556861815866No Hit
GTGTTAATACGTGAGACCCACACCAGCCCCTCCCCTGGAGCTTGGCGGAC9590.13689087841406422No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9550.13631990499002225No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9480.13532070149794878No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9420.1344642413618858No Hit
TCCAGGGACTATCATAATATTGCTGACAGTAATAAACTGCCACATCTTCA9340.13332229451380187No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA9180.13103840081763396No Hit
AACCAGGACAGCCTCCTAAATTGCTCATTTATTGGGCATCTACCCGGGAC9010.12861176376545552No Hit
GTCTTAGAGCCGAGGACACGGCTGTGTATTATTGTGCAAGGAGGGGGTTC8960.12789804698540305No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG8900.12704158684934008No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8820.12589964000125614No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA8670.12375848966109872No Hit
GTCAGGGACCCCGGAGTCCCGGGTAGATGCCCAATAAATGAGCAATTTAG8530.12176008267695179No Hit
CAATAATACACAGCCGTGTCCTCGGCTCTAAGACTGCTCATCTGCAGATA8510.1214745959649308No Hit
CCTTTAGTAGTTACTGGATGCACTGGGTCCGCCAAGCTCCAGGGGAGGGG8430.12033264911684685No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC8410.12004716240482587No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8400.11990441904881537No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT7890.11262450789228015No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC7810.1114825610441962No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT7800.11133981768818571No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7740.11048335755212274No Hit
ATACACAGCCGTGTCCTCGGCTCTAAGACTGCTCATCTGCAGATATAGGG7700.10991238412808077No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG7680.10962689741605977No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC7600.10848495056797582No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7560.10791397714393385No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT7540.10762849043191286No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC7520.10734300371989186No Hit
TTTAAAAGGTGTCCAGTGTGTGGTGCAGCTGGTGGAGTCCGGGGGAGGCT7440.1062010568718079No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7340.10477362331170297No Hit
CCCTATATCTGCAGATGAGCAGTCTTAGAGCCGAGGACACGGCTGTGTAT7330.10463087995569247No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA7310.10434539324367148No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA7300.10420264988766098No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7290.1040599065316505No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA7280.10391716317564No Hit
GTGTATTATTGTGCAAGGAGGGGGTTCCGGGGGGCTGCACTTGATATCTG7270.10377441981962951No Hit
ATGATAGTCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA7250.10348893310760851No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAATTGCTCA7220.10306070303957704No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTA7220.10306070303957704No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7200.10277521632755604No Hit
TACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCT7190.10263247297154555No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG7180.10248972961553506No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT7100.10134778276745107No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT7060.1007768093434091No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC7040.10049132263138812No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)