FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700558
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT32820.46848369442644294No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24830.3544317529740578No Hit
ATATTATGATAGTCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA23130.3301653824522738No Hit
GTTTTATACAGCTCCACCAATAACAACTACTTAGCTTGGTACCAGCAGAA22810.32559759505993796No Hit
ATCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGC21040.3003320210460804No Hit
ATAATACACAGCCGTGTCCTCGGCTCTAAGACTGCTCATCTGCAGATATA19570.2793487477125377No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18710.2670728190956352No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17860.25493963383474316No Hit
TATTATGATAGTCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAA17680.2523702534265542No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17300.24694600589815546No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16990.24252096186183014No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16010.22853211297280168No Hit
TTATTACTGTCAGCAATATTATGATAGTCCCTGGACGTTCGGCCAAGGGA15340.21896830812009857No Hit
GTTTATTACTGTCAGCAATATTATGATAGTCCCTGGACGTTCGGCCAAGG14460.20640689279117505No Hit
GTATAAAACATTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA14450.20626414943516455No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT13990.19969795505868176No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13910.19855600821059782No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA13730.1959866278024089No Hit
GTCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTG13720.19584388444639841No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13520.19298901732618856No Hit
TCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT13380.19099061034204165No Hit
ATTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTGTTA13100.18699379637374777No Hit
GCTCTAAGACTGCTCATCTGCAGATATAGGGTGTTCTTGGCGTTGTCTCT12990.18542361945763233No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12930.18456715932156936No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12590.17971388521721257No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12140.1732904341967403No Hit
GATATAGGGTGTTCTTGGCGTTGTCTCTGGAGATGATAAATCGGCCCTTC12080.17243397406067734No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12010.1714347705686039No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11910.17000733700849893No Hit
TCCAGGGACTATCATAATATTGCTGACAGTAATAAACTGCCACATCTTCA11440.1632983992760057No Hit
TCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG11340.16187096571590076No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC11150.15915884195170135No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11150.15915884195170135No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11060.1578741517476069No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10990.15687494825553344No Hit
TATTAACACTGATGGGACTACCAAAAGTTACGCGGACTCCGTGAAGGGCC10750.15344910771128156No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10480.1495950370989982No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT10440.14902406367495624No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10290.14688291333479883No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10280.14674016997878833No Hit
GTGTTAATACGTGAGACCCACACCAGCCCCTCCCCTGGAGCTTGGCGGAC10230.14602645319873586No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG10180.1453127364186834No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10140.14474176299464142No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA9960.1421723825864525No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9820.1401739756023056No Hit
GTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACCCCGGAGTCCCGGGT9480.13532070149794878No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9460.1350352147859278No Hit
CTCCTAAATTGCTCATTTATTGGGCATCTACCCGGGACTCCGGGGTCCCT9400.13417875464986484No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9350.13346503786981237No Hit
GTCCCTGACCGATTCACTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC9250.1320376043097074No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9220.13160937424167593No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9170.13089565746162346No Hit
ACCCGGGACTCCGGGGTCCCTGACCGATTCACTGGCAGCGGGTCTGGGAC9160.13075291410561296No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9050.1291827371894975No Hit
GTCTTAGAGCCGAGGACACGGCTGTGTATTATTGTGCAAGGAGGGGGTTC9040.12903999383348702No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9030.12889725047747652No Hit
GTCAGGGACCCCGGAGTCCCGGGTAGATGCCCAATAAATGAGCAATTTAG9020.12875450712146602No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8970.12804079034141355No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG8850.1263278700692876No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC8750.12490043650918267No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC8740.12475769315317217No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8660.12361574630508823No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT8640.12333025959306723No Hit
CAATAATACACAGCCGTGTCCTCGGCTCTAAGACTGCTCATCTGCAGATA8190.11690680857259499No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCC8180.1167640652165845No Hit
CCTTTAGTAGTTACTGGATGCACTGGGTCCGCCAAGCTCCAGGGGAGGGG8070.11519388830046906No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC8000.11419468480839559No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT7990.1140519414523851No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7850.11205353446823818No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA7770.11091158762015423No Hit
ATACACAGCCGTGTCCTCGGCTCTAAGACTGCTCATCTGCAGATATAGGG7740.11048335755212274No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7590.10834220721196533No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC7590.10834220721196533No Hit
CCCTATATCTGCAGATGAGCAGTCTTAGAGCCGAGGACACGGCTGTGTAT7530.10748574707590236No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTA7390.10548734009175544No Hit
AACCAGGACAGCCTCCTAAATTGCTCATTTATTGGGCATCTACCCGGGAC7360.10505911002372395No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG7320.10448813659968198No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC7310.10434539324367148No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7180.10248972961553506No Hit
GTGTATTATTGTGCAAGGAGGGGGTTCCGGGGGGCTGCACTTGATATCTG7100.10134778276745107No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA7020.10020583591936712No Hit
TTTAAAAGGTGTCCAGTGTGTGGTGCAGCTGGTGGAGTCCGGGGGAGGCT7020.10020583591936712No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTGTTATTGGTGGAGCTG7020.10020583591936712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATA806.129958E-754.0362661
GAATACG1755.820766E-1141.135219
CAGCGCT3650.039.4447149
GCAGCGC3650.039.4447148
GTGCTAG2250.038.425791
GTACACG7200.037.011625
TACACGG7300.036.4837576
GTCTAAT800.00209961736.0241781
TATGTAC7600.036.0216032
ACGGGGT7700.035.525869
GTATGTA8350.035.3770371
ACACGGG7700.034.5884977
CACGGGG8250.033.1574678
ATAGCGG900.003742825732.016913
ATAGTAC1453.644487E-529.8088513
TCAACGC17100.029.4871564
GCTAGAG2202.4072506E-829.4701143
TGCTAGA2951.6370905E-1129.305712
ATCAACG17300.029.1483443
CAACGCA17650.028.972275
Produced by FastQC (version 0.11.5)