FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539472
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8100.1501468102144319No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6260.11603938665954859No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA400.00621876453.9967356
GTATCAA20700.038.35811
ATCAACG22100.035.8349343
TATCAAC22800.034.7734152
TCAACGC22850.034.6587334
CAACGCA23250.034.0624545
AACGCAG24050.032.92946
CGCAGAG24450.032.390688
ACGCAGA24600.032.1931727
GTACGGC900.00370542132.081321
GCAGAGT27750.028.5388139
ATAGTAC2553.250534E-928.2335873
TATACTG3103.092282E-1127.8692825
CTAGGTC4100.026.3398693
CTAGCTC2259.665346E-725.5984523
TAGACGG3701.0913936E-1125.2957655
GTTACAC2001.1234877E-525.1984753
GTATAGT3251.5588739E-924.4311621
CTAGCAC2101.6396149E-523.9985473
TAGGTCA4850.023.751144
Produced by FastQC (version 0.11.5)