FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539472
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14200.030.4535311
TCAACGC13050.029.7875214
ATCAACG13150.029.0135773
TATCAAC14350.027.590652
CAACGCA14500.026.808775
CTCACGG1400.001041155425.7094692
TCTACAC2851.0650183E-825.2584273
AACGCAG15250.025.0182656
GTATTAT3751.2732926E-1124.9854321
CGCAGAG15200.024.6269658
ACGCAGA15250.024.546227
TAGACTA2202.352094E-522.90485
GTCTATA3153.043533E-822.8804321
CATGGGG9450.022.8528614
CTAGACT2552.875353E-622.5840034
TAGGACG3854.656613E-1022.4373555
GTACATG20000.021.9823741
CCCCTAT4607.2759576E-1221.935371
ACGTGAG3305.045331E-821.8140967
TTAGACT2353.9138733E-521.4427914
Produced by FastQC (version 0.11.5)