FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297016
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC43870.3382379245899819No Hit
GTCTATATGGAACTGAGCAGCCTGAGATCTGACGACACGGCCGTGTATTA38390.2959870965354321No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC38330.2955244962282655No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA37180.2866579903409056No Hit
CCCCTGTACTTGTCATCTATTCTAAAACCAACCGGCCCTCAGGGATCCCA35180.27123798010201877No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT35080.27046697959007443No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA33930.26160047370271455No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGTCCCAGTGATGGTCAAGGAAG33170.25574086981193755No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32850.25327366817371566No Hit
GACCTAGGACGGTGACCTTGGTCCCAGGTCCGAAGACATAAAGGGGGTCA32420.24995836597235502No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT31720.24456136238874462No Hit
ACCTAGGACGGTGACCTTGGTCCCAGGTCCGAAGACATAAAGGGGGTCAC31440.24240256095530047No Hit
CTCTTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA31350.2417086604945506No Hit
ATATGGAACTGAGCAGCCTGAGATCTGACGACACGGCCGTGTATTACTGT30010.2313772536344964No Hit
GACTATTACTGTAGCTCCCGGGACAACAGTGGTGACCCCCTTTATGTCTT29670.22875585189388567No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC28000.2158801433444152No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC27820.21449234242291534No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC27760.21402974211574877No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCT27160.20940373904408272No Hit
AGCCAGGACAGGCCCCTGTACTTGTCATCTATTCTAAAACCAACCGGCCC25130.19375242865161263No Hit
CTATTACTGTAGCTCCCGGGACAACAGTGGTGACCCCCTTTATGTCTTCG25000.19275012798608498No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG24760.19089972675741856No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGTTCCATATAGA23600.18195612081886423No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG23600.18195612081886423No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCTTCACTCTTTGCATAGGTTCTG23510.18126222035811432No Hit
TTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGT23510.18126222035811432No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGCGACAGCC22450.17308961493150432No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC22380.17254991457314328No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21650.16692161083594959No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA21470.1655338099144498No Hit
GTGTATTACTGTGTGAGAGGTTGTAGTCCGACCGTCTGCATCAACTTTGA20580.15867190535814515No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT20540.15836350515336742No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT20520.15820930505097855No Hit
CATATAGACTGTGCTCGTGGACGTGTCCCTGGTCACGGTGACTCTGCCCT20360.1569757042318676No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC20040.15450850259364574No Hit
ATTCTAAAACCAACCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCC19930.15366040203050696No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG19550.15073060008511846No Hit
GTACTTGTCATCTATTCTAAAACCAACCGGCCCTCAGGGATCCCAGACCG19450.14995959957317412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19440.14988249952197968No Hit
GTACATGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCA19400.14957409931720195No Hit
GTACAGGGGCCTGTCCTGGCTTCTGCCGGAACCAGCTTGTATAATCGCTT19170.14780079813972996No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGTCCCAGTGATGGTCAAGGA18380.1417098940953697No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18280.14093889358342535No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCTTCACTCTTTG18040.13908849235475892No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG17930.13824039179162015No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17730.13669839076773146No Hit
GTCAGGATCACATGCCAAGGCGACAGCCTCAGAAGCGATTATACAAGCTG17730.13669839076773146No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17570.13546478994862052No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT17350.133768588822343No Hit
CTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACA17170.13238078790084315No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16890.130221986467399No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16880.13014488641620456No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG16350.12605858370289957No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG16150.12451658267901089No Hit
ATACAAGCTGGTTCCGGCAGAAGCCAGGACAGGCCCCTGTACTTGTCATC16060.123822682218261No Hit
CCTTTATGTCTTCGGACCTGGGACCAAGGTCACCGTCCTAGGTCAGCCCA15810.12189518093840014No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15680.1208928802728725No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC15540.11981347955615043No Hit
CGATTATACAAGCTGGTTCCGGCAGAAGCCAGGACAGGCCCCTGTACTTG15390.11865697878823392No Hit
CACTGGGACTCAGGCGGAAGATGAGGCTGACTATTACTGTAGCTCCCGGG15300.11796307832748401No Hit
GTATTACTGTGTGAGAGGTTGTAGTCCGACCGTCTGCATCAACTTTGACT15220.11734627791792854No Hit
CCTCAGAAGCGATTATACAAGCTGGTTCCGGCAGAAGCCAGGACAGGCCC15200.11719207781553968No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15120.1165752774059842No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG14850.11449357602373447No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGGTCCGAAGACATA14800.11410807576776232No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14750.11372257551179013No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14680.1131828751534291No Hit
GTTCCATATAGACTGTGCTCGTGGACGTGTCCCTGGTCACGGTGACTCTG14560.1122576745390959No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT14510.11187217428312374No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC14440.11133247392476268No Hit
ACATAAAGGGGGTCACCACTGTTGTCCCGGGAGCTACAGTAATAGTCAGC14290.11017597315684617No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14200.10948207269609626No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14180.1093278725937074No Hit
GTATCAACGCAGAGTACATGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGG14160.10917367249131853No Hit
TTCTAAAACCAACCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCA14000.1079400716722076No Hit
GTTGTAGTCCGACCGTCTGCATCAACTTTGACTTCTGGGGCCAGGGAACC13640.10516446982920796No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCC13590.10477896957323579No Hit
TCCTTGACCATCACTGGGACTCAGGCGGAAGATGAGGCTGACTATTACTG13460.10377666890770815No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG13170.10154076742306957No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAAC13110.10107816711590296No Hit
GTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCC13050.10061556680873636No Hit
GCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCAGCTCAGGAAACACAG13010.10030716660395862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)