FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297016
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATATGGAACTGAGCAGCCTGAGATCTGACGACACGGCCGTGTATTA38710.298454298173654No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT35670.27501588261054605No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC35370.272702881074713No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC34090.2628340745218255No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA34070.26267987441943663No Hit
CCCCTGTACTTGTCATCTATTCTAAAACCAACCGGCCCTCAGGGATCCCA33380.2573599708870207No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGTCCCAGTGATGGTCAAGGAAG33000.2544301689416322No Hit
GACCTAGGACGGTGACCTTGGTCCCAGGTCCGAAGACATAAAGGGGGTCA31240.2408605599314118No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA30720.23685135726930123No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA30660.2363887569621346No Hit
CTCTTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA29640.22852455174030237No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT28300.21819314488024819No Hit
ACCTAGGACGGTGACCTTGGTCCCAGGTCCGAAGACATAAAGGGGGTCAC27590.2127190412454434No Hit
GACTATTACTGTAGCTCCCGGGACAACAGTGGTGACCCCCTTTATGTCTT26440.2038525353580835No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCT26400.20354413515330572No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC26260.20246473443658367No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC25790.19884103203044526No Hit
ATATGGAACTGAGCAGCCTGAGATCTGACGACACGGCCGTGTATTACTGT25650.1977616313137232No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGTTCCATATAGA25630.19760743121133434No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC24400.18812412491441896No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22750.17540261646733735No Hit
AGCCAGGACAGGCCCCTGTACTTGTCATCTATTCTAAAACCAACCGGCCC22590.1741690156482264No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG22360.1723957144707544No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA22110.17046821319089356No Hit
CTATTACTGTAGCTCCCGGGACAACAGTGGTGACCCCCTTTATGTCTTCG21870.16861781196222714No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCTTCACTCTTTGCATAGGTTCTG21380.16483990945369986No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC21130.162912408173839No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG21110.16275820807145014No Hit
TTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGT21060.162372707815478No Hit
CATATAGACTGTGCTCGTGGACGTGTCCCTGGTCACGGTGACTCTGCCCT20800.1603681064844227No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGCGACAGCC20450.1576696046926175No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19960.15389170218409023No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT19510.15042219988034072No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT19340.14911149901003534No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC19170.14780079813972996No Hit
GTGTATTACTGTGTGAGAGGTTGTAGTCCGACCGTCTGCATCAACTTTGA18760.1446396960407582No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG18720.14433129583598042No Hit
GTACTTGTCATCTATTCTAAAACCAACCGGCCCTCAGGGATCCCAGACCG18450.14224959445373073No Hit
ATTCTAAAACCAACCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCC18410.14194119424895296No Hit
GTACATGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCA17550.13531058984623165No Hit
GTACAGGGGCCTGTCCTGGCTTCTGCCGGAACCAGCTTGTATAATCGCTT17310.13346018861756523No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCTTCACTCTTTG17260.13307468836159306No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17090.1317639874912877No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17010.1311471870817322No Hit
GTTCCATATAGACTGTGCTCGTGGACGTGTCCCTGGTCACGGTGACTCTG16770.1292967858530658No Hit
GTCAGGATCACATGCCAAGGCGACAGCCTCAGAAGCGATTATACAAGCTG16550.12760058472678826No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16450.12682958421484392No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGTCCCAGTGATGGTCAAGGA16380.12628988385648288No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG15890.1225119813479556No Hit
CTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACA15490.11942797930017826No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15380.11857987873703948No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT14930.11511037643328996No Hit
CCTTTATGTCTTCGGACCTGGGACCAAGGTCACCGTCCTAGGTCAGCCCA14870.11464777612612334No Hit
GTATTACTGTGTGAGAGGTTGTAGTCCGACCGTCTGCATCAACTTTGACT14780.11395387566537345No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC14600.11256607474387362No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14580.11241187464148476No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14450.11140957397595712No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA14370.11079277356640166No Hit
ATACAAGCTGGTTCCGGCAGAAGCCAGGACAGGCCCCTGTACTTGTCATC14330.11048437336162391No Hit
CGATTATACAAGCTGGTTCCGGCAGAAGCCAGGACAGGCCCCTGTACTTG14270.1100217730544573No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG14170.10925077254251296No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGGTCCGAAGACATA14010.10801717172340203No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT13840.10670647085309665No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG13820.10655227075070778No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG13740.1059354703411523No Hit
CCTCAGAAGCGATTATACAAGCTGGTTCCGGCAGAAGCCAGGACAGGCCC13710.105704170187569No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13690.10554997008518013No Hit
GTATCAACGCAGAGTACATGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGG13680.10547287003398571No Hit
TTCTAAAACCAACCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCA13630.10508736977801353No Hit
ACATAAAGGGGGTCACCACTGTTGTCCCGGGAGCTACAGTAATAGTCAGC13460.10377666890770815No Hit
CACTGGGACTCAGGCGGAAGATGAGGCTGACTATTACTGTAGCTCCCGGG13390.10323696854934712No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13370.10308276844695825No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG12980.10007586645037533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17700.061.825761
ATCAACG19400.055.215733
TCAACGC19400.054.8472754
GGTATCA6750.054.395821
TATCAAC21000.051.6934972
CAACGCA21600.049.9363065
AACGCAG22500.047.9481126
CGCAGAG25050.043.0671658
GTGCTAG4500.041.59681
GTACATG39000.039.3200571
TACATGG38650.039.0614282
ACATGGG39150.038.5625573
GAATACG4150.036.394359
ACGCAGA30750.035.319247
ATACCGT1551.5599526E-632.4809766
ATGGGAG15300.032.4292265
CTAGAGA6150.031.5633954
CATGGGA21750.031.4021514
TATGTAC12950.031.0882682
GGATATT8350.031.0395341
Produced by FastQC (version 0.11.5)