FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096235
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20770.1894666745725141No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19870.1812567560787605No Hit
ATAATAGACAGCCATGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATACA19850.18107431344556596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15150.13820029464485262No Hit
ATTATGGCCTCCCTCGGACTTTCGGCGGTGGGACCAAGGTGGACCGCAGA14430.13163235984984972No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13100.1194999247424138No Hit
GTCTATTATTGTGTGACTTCTGGTGGGAGCTACTCTGAGTACTGGGGCCA12810.11685450656109321No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11370.1037186369710874No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11330.10335375170469835No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11110.10134688273955858No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11030.10061711220678048No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG22900.064.181313
TCAACGC23150.063.1774
CAACGCA23450.062.368765
AACGCAG25450.057.4648556
CGCAGAG29450.049.38368
GTATACG752.6442403E-548.0398831
GTATCAA33200.045.1459121
GCAGAGT35050.041.2880829
TATACGG700.001089799841.169532
TATCAAC37700.039.1765752
GTACACG5750.033.830615
ACGCAGA43550.033.5755167
TACACGG5950.032.6919566
GTACATG25150.032.090261
TACATGG25050.030.4868142
ACACGGG6150.030.4518057
ACATGGG24950.030.3202423
GGTATCA20700.027.8492091
ACTGTCG1306.752479E-427.6912988
CATGGGA14250.027.3018954
Produced by FastQC (version 0.11.5)