FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096235
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19130.17450637865056307No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19010.17341172285139592No Hit
ATAATAGACAGCCATGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATACA18090.16501936172444776No Hit
ATTATGGCCTCCCTCGGACTTTCGGCGGTGGGACCAAGGTGGACCGCAGA13860.12643274480380576No Hit
GTCTATTATTGTGTGACTTCTGGTGGGAGCTACTCTGAGTACTGGGGCCA13220.12059458054158095No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12350.11265832599761912No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11780.10745871095157516No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11140.10162054668935037No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG12250.047.661973
TCAACGC12750.045.790784
CAACGCA13850.041.629745
AACGCAG14800.039.4139026
CGCAGAG17550.032.8305938
GTACATG26650.027.3316381
TACATGG27300.026.9327342
ACATGGG27150.026.0183223
GTATCAA23750.025.8105261
TACACGG6300.025.148286
GCAGAGT22800.024.953899
CGCAGTA1450.00126828524.8567832
CAGCGCT4700.024.5170449
GTACACG6650.023.8431995
TATCAAC25400.023.8390242
GGCAGCG4850.023.759877
ACACGGG7050.022.4749837
ACTATAC5500.022.2796973
CTATACA4007.421477E-1021.6234234
GTATATA8100.021.368191
Produced by FastQC (version 0.11.5)