FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644680
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCGACTTCCAGGCTGCTCATTTGCAGATACA31200.48396103493205933No Hit
ATATATTACTGTGCGACAGTTCCAATTTCTTGCAGTAGTAGTCTCTGCTA28730.44564745299993797No Hit
ATACTATGCTGACCCCGTGAAGGGCCGCTTCACCATCTCCAGAGATAATT21090.32713904572811314No Hit
GCCATATATTACTGTGCGACAGTTCCAATTTCTTGCAGTAGTAGTCTCTG15330.23779239312527145No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15240.23639635167835207No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15050.2334491530681889No Hit
ATATATGGCCGTGTCCTCGACTTCCAGGCTGCTCATTTGCAGATACAGCG15000.232673574486567No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14150.21948873859899484No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13840.21468015139293914No Hit
GCATAGTATGTGCTGCTACCACCCCTATAAATAACTGAGACCCACTCCAG12790.19839300117887945No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12290.19063721536266054No Hit
GTGTAGCAGAGACTACTACTGCAAGAAATTGGAACTGTCGCACAGTAATA11880.18427747099336106No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11770.1825711981137929No Hit
ATATTACTGTGCGACAGTTCCAATTTCTTGCAGTAGTAGTCTCTGCTACA11740.18210585096481977No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11380.17652168517714215No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11360.1762114537444934No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10270.1593038406651362No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10030.1555810634733511No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9620.14922131910405162No Hit
CTGCTACACAGGGAATACAGCCCTGGCTGCGGAGTGGGGCCAGGGAACCC9320.1445678476143203No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8880.13774275609604764No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8740.13557113606750634No Hit
GGACTGAACAGAGAGGACTTACCATGGAGTTTGGGCTGAGCTGGCTTTTT8600.13339951603896508No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8450.13107278029409938No Hit
CAGTAATATATGGCCGTGTCCTCGACTTCCAGGCTGCTCATTTGCAGATA8260.12812558168393623No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8250.12797046596761183No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8030.12455792020847553No Hit
GCACATACTATGCTGACCCCGTGAAGGGCCGCTTCACCATCTCCAGAGAT7950.1233169944778805No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG7860.12192095303096111No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTTAGTAACTCTGCCATGAGTTGGG7820.12130049016566358No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGTTTTAGTAACTCTGCC7800.12099025873301483No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAACTGTTGGAGTCTGGGGGAAGCT7780.12068002730036607No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA7770.12052491158404169No Hit
GTAGTAGTCTCTGCTACACAGGGAATACAGCCCTGGCTGCGGAGTGGGGC7760.12036979586771732No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7660.11881863870447354No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT7650.11866352298814915No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC7450.11556120866166161No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7320.11354470434944469No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT7020.10889123285971335No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA6980.10827076999441584No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC6940.10765030712911831No Hit
GTATTCCCTGTGTAGCAGAGACTACTACTGCAAGAAATTGGAACTGTCGC6920.10734007569646957No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG6870.10656449711484767No Hit
ATGCTGACCCCGTGAAGGGCCGCTTCACCATCTCCAGAGATAATTCCAAG6840.10609914996587454No Hit
AGTATGTGCTGCTACCACCCCTATAAATAACTGAGACCCACTCCAGCCCC6510.10098033132717008No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)