FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644680
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCGACTTCCAGGCTGCTCATTTGCAGATACA31430.48752869640752No Hit
ATATATTACTGTGCGACAGTTCCAATTTCTTGCAGTAGTAGTCTCTGCTA28040.43494446857355584No Hit
ATACTATGCTGACCCCGTGAAGGGCCGCTTCACCATCTCCAGAGATAATT21260.3297760129056276No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19890.3085251597691878No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15690.24337655891294904No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14770.22910591301110628No Hit
GCCATATATTACTGTGCGACAGTTCCAATTTCTTGCAGTAGTAGTCTCTG14300.22181547434386054No Hit
ATATATGGCCGTGTCCTCGACTTCCAGGCTGCTCATTTGCAGATACAGCG13720.21281876279704662No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12870.19963392690947446No Hit
GCATAGTATGTGCTGCTACCACCCCTATAAATAACTGAGACCCACTCCAG12790.19839300117887945No Hit
GTGTAGCAGAGACTACTACTGCAAGAAATTGGAACTGTCGCACAGTAATA11960.18551839672395606No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11690.18133027238319788No Hit
ATATTACTGTGCGACAGTTCCAATTTCTTGCAGTAGTAGTCTCTGCTACA11630.1803995780852516No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11460.17776261090773718No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11440.1774523794750884No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10280.15945895638146057No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10010.15527083204070236No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9890.15340944344480983No Hit
CTGCTACACAGGGAATACAGCCCTGGCTGCGGAGTGGGGCCAGGGAACCC9440.1464292362102128No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9370.14534342619594218No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9080.14084507042253522No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTTAGTAACTCTGCCATGAGTTGGG9020.13991437612458896No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT8790.13634671464912826No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8730.135416020351182No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8580.1330892846063163No Hit
CAGTAATATATGGCCGTGTCCTCGACTTCCAGGCTGCTCATTTGCAGATA8530.13231370602469442No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8320.1290562759818825No Hit
GCACATACTATGCTGACCCCGTGAAGGGCCGCTTCACCATCTCCAGAGAT8150.12641930880436808No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8100.12564373022274616No Hit
ATGCTGACCCCGTGAAGGGCCGCTTCACCATCTCCAGAGATAATTCCAAG7830.12145560588198796No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7820.12130049016566358No Hit
GGACTGAACAGAGAGGACTTACCATGGAGTTTGGGCTGAGCTGGCTTTTT7800.12099025873301483No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG7770.12052491158404169No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC7640.11850840727182478No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAACTGTTGGAGTCTGGGGGAAGCT7550.11711236582490539No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGTTTTAGTAACTCTGCC7440.11540609294533723No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG7430.11525097722901284No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT7350.11401005149841781No Hit
GTATTCCCTGTGTAGCAGAGACTACTACTGCAAGAAATTGGAACTGTCGC7300.11323447291679593No Hit
GTAGTAGTCTCTGCTACACAGGGAATACAGCCCTGGCTGCGGAGTGGGGC7070.10966681144133524No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG6830.10594403424955016No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA6800.10547868710057703No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA6740.10454799280263077No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT6520.10113544704349443No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6490.10067009989452132No Hit
GATCAGGACTGAACAGAGAGGACTTACCATGGAGTTTGGGCTGAGCTGGC6480.10051498417819693No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC6450.10004963702922381No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT6450.10004963702922381No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAG350.003658000161.7442321
CTAAGGC1054.0033774E-641.150043
ATCAACG11000.038.6249283
TACACGG6750.038.3947876
TATCAAC11300.037.5994832
GTACACG6900.037.568865
TCAACGC11300.036.9593354
CAACGCA11600.036.003495
GTATCAA11900.035.7147981
AACGCAG11750.035.53566
GGCAGCG3450.035.4734427
CGCAGAG12150.034.3657048
ACACGGG7750.033.440627
GTATTAG1957.4360287E-933.2468951
GTATGTA8800.032.7431531
GTACTAA1352.2373313E-532.0155261
CAGCGCT3900.031.3803549
ATGGCCG16650.030.6985348
TATGTAC8450.030.6799132
AAGGCCG1652.5150075E-630.5484185
Produced by FastQC (version 0.11.5)