FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073358
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAAATCTCAGGCTGCTCAAGTCCATGTAGG49820.46415082386305406No Hit
GTCTATTACTGTGCGACAAGGGGGTTTGGGGCAGCTGACCCGTACTGGTA37550.3498366807719326No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24240.2258333193584992No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGATCTCCTGCA22500.20962251178078517No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC22240.2072002072002072No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21890.20393941257250608No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT21780.20291459140380003No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20470.19070990294011878No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC20290.18903292284587248No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAACGTGGCTG19100.17794622111168873No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18880.1758965787742766No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT18530.17263578414657552No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17710.1649962081616758No Hit
GATTATTACTGCTGCTCATATGCAGGCAGCCACGTTTATGTCTTCGGAAC17390.16201491021634906No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT17250.1607105923652686No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17140.15968577119656258No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA17080.1591267778318138No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG16640.15502749315698955No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAACGTGGCTGC16620.15484116203540665No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG15700.14626993044259232No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC15420.14366129474043143No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG15160.14123899015985347No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT15120.14086632791668763No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15070.14040050011273034No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA14600.1360217187555317No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14500.1350900631476171No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC14240.13266775856703913No Hit
TTATAACTATGTCTCCTGGTACCGACAACACCCAGGCAAAGCCCCCAAAC14240.13266775856703913No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG14220.13248142744545624No Hit
GCCTACATGGACTTGAGCAGCCTGAGATTTGAGGACACGGCCGTCTATTA14060.13099077847279286No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13830.1288479705745893No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT13000.12111522902889808No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTAATTATAACTATGTCTCCT12880.11999724229940058No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12770.1189724211306945No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12230.11394148084795566No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT12220.1138483152871642No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG12020.11198500407133502No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG12000.11179867294975208No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11970.1115191762673777No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11800.10993536173392288No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11610.10816521607888514No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG11530.10741989159255345No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11400.10620873930226447No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11130.10369326916089507No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGAATCATCCCTGTCTTTGGT10960.10210945462744024No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10920.1017367923842744No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC10830.10089830233715126No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG10740.10005981229002812No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10100.062.0198821
TCAACGC10900.056.1468624
ATCAACG10650.056.1127473
CAACGCA11400.054.947445
TATCAAC12000.050.4000662
AACGCAG12500.050.1050646
TAACGGC450.009911947.993358
CGCAGAG15000.040.794358
GGTATCA4600.036.0000461
GTACATG22800.033.789521
TAGACGG20400.031.764755
ATAGACG21250.031.510634
AGACGGC21250.030.4898976
GTAATAG22950.030.4314141
CAGAGTA17750.030.4183249
AATAGAC21950.029.8496973
ACGCAGA20650.029.6327027
ACATGGG24600.029.5610143
CAGCGCT7350.029.3836849
TAATAGA22750.029.116522
Produced by FastQC (version 0.11.5)