FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867333
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT30840.35557277308715335No Hit
ATATATTACTGTGCGAAGTGCAGTTCGTCCTGGGGGAATGATGCCCTTGA30140.34750205515067456No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACA28690.33078413942511126No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24060.2774021050738298No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT19900.22943898133704127No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA18850.21733290443232298No Hit
GCCATATATTACTGTGCGAAGTGCAGTTCGTCCTGGGGGAATGATGCCCT17600.2029209081171822No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT16090.18551121656849215No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15850.18274411327598514No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15060.17363573160481613No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT14350.16544971769781616No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT14150.16314379828739364No Hit
ATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACAGAG13900.1602613990243655No Hit
ATATCACACTATTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA13720.15818607155498524No Hit
GCTTAGGAGGGTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTGTCT12930.14907768988381623No Hit
ATATTACTGTGCGAAGTGCAGTTCGTCCTGGGGGAATGATGCCCTTGATG12850.14815532211964724No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12730.14677177047339374No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG11900.13720220492014024No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11080.1277479353374079No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11070.12763263936688676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11040.12728675145532337No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11000.1268255675732389No Hit
TTATTACTGTCAGCAATATCACACTATTCCGTGGACGTTCGGCCAAGGGA10840.12498083204490087No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10180.11737129799050652No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG9810.11310534708122486No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA9740.11229827528757698No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCGATTTTGCCATGAACTGGG9430.10872410020142206No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA9280.10699466064360517No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9240.10653347676152067No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG9200.10607229287943616No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC9140.1053805170563094No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9060.1044581492921404No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9020.10399696541005589No Hit
ACACTATTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA8900.10261341376380237No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA8770.10111456614702771No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18450.060.2347371
ATCAACG20400.053.289513
TCAACGC20850.051.7940864
TATCAAC21500.050.6039122
CAACGCA21250.050.4803435
AACGCAG22550.047.5701686
GGTATCA8500.044.996591
CGCAGAG25150.042.9386378
GTACACG7100.037.5178835
GCAGAGT29350.037.0393879
TACACGG7500.035.516936
GTATGTA8750.034.638881
GTACATG23150.033.6663061
TATGTAC8250.032.3141982
ACGGGGT8700.031.4455579
TACATGG24800.031.0869182
CCTAAGC17550.029.9461293
ACATGGG25250.029.9380043
ACACGGG9100.029.2721947
ATGGCCG15200.028.8922378
Produced by FastQC (version 0.11.5)