FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867333
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATTACTGTGCGAAGTGCAGTTCGTCCTGGGGGAATGATGCCCTTGA26030.3001154112664916No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACA24140.27832447283799877No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT21230.24477334541635104No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19640.22644128610349196No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT16950.19542667003330902No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA16370.18873950374308368No Hit
GCCATATATTACTGTGCGAAGTGCAGTTCGTCCTGGGGGAATGATGCCCT14130.16291320634635142No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT12880.1485012100312106No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12690.14631058659130922No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12660.14596469867974585No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT12120.13973871627160503No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11790.13593394924440785No Hit
ATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACAGAG11660.13443510162763322No Hit
ATATCACACTATTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA11510.13270566206981632No Hit
GCTTAGGAGGGTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTGTCT11080.1277479353374079No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA10240.11806307381363329No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT10100.11644893022633752No Hit
ATATTACTGTGCGAAGTGCAGTTCGTCCTGGGGGAATGATGCCCTTGATG10020.1155265624621685No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG9530.10987705990663332No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9300.10722525258464741No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9300.10722525258464741No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9010.10388166943953475No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8940.10307459764588686No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG10800.048.6580163
TCAACGC10750.048.2146874
GTATCAA11700.048.0385481
AACGCAG12700.041.378476
CAACGCA12300.040.9683275
TATCAAC13250.040.747472
CCCGATC750.00153592538.3931779
GTACATG21050.037.3126031
TACATGG21350.036.752252
CGCAGAG14350.035.617368
ACATGGG21650.034.9129643
GAATACG1905.81349E-934.09929
TAAACGA850.00283887233.8763285
GTGCTAG3200.033.7771031
GTAATAT16150.033.0172041
GGTATCA6700.032.2646941
CAGTGCG1153.2961377E-431.2987849
ATGGCCG15400.030.8516588
ATATGGC16500.029.6674546
TATGGCC16200.029.328127
Produced by FastQC (version 0.11.5)