FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489893
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30770.12357960763775794No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41300.068.025991
ATCAACG45850.060.3071823
TCAACGC46200.060.006174
CAACGCA48450.056.919975
AACGCAG49650.055.6859366
GGTATCA22650.050.5695721
TATCAAC55100.050.444392
ACGCAGA58500.047.26077
CGCAGAG64950.042.4565248
GCAGAGT81450.033.944159
ACATGGG70550.028.476353
GTACATG83750.027.7829321
TACATGG88300.025.6062552
CAGAGTA75000.023.9035789
CCTATAC9650.021.639533
GTACTAG5800.021.1145231
TATACCG2053.842443E-421.0744925
GTATTAG14050.020.5089781
CATGGGA36700.019.424344
GTATAGG9450.019.0576151
Produced by FastQC (version 0.11.5)