FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250922
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTACTGTGTGAGGTTGGGTGGAAGTAGGACTGAAAACTGGGGCCA41070.3283178327665514No Hit
GTATTACTGTGTGAGGTTGGGTGGAAGTAGGACTGAAAACTGGGGCCAGG33860.2706803461766601No Hit
GTAATACATAGCCGTGTCCTCGGCTCGCAGGCTCCTCATGTGCAGATACA32060.2562909597880603No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27270.2179992037872865No Hit
TATAAAAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT25440.20336999429221006No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23630.18890066686811807No Hit
CAGTAATACATAGCCGTGTCCTCGGCTCGCAGGCTCCTCATGTGCAGATA22820.18242544299324817No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC21420.17123369802433724No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20560.16435876897200624No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19480.15572513713884636No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18900.15108855708029756No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18820.1504490287963598No Hit
ATATTACTGTCAACACTATGATAGTCTCCCTCGGACGTTCGGCCAAGGGA17890.14301451249558325No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17810.1423749842116455No Hit
CTATGATAGTCTCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGACATCA17780.14213516110516883No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16800.13430093962693118No Hit
GTGTGAGGTTGGGTGGAAGTAGGACTGAAAACTGGGGCCAGGGAATCCTG15770.1260670129712324No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG15380.12294931258703581No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15370.12286937155154358No Hit
GTACATGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGT15330.12254960740957471No Hit
GCTATGTATTACTGTGTGAGGTTGGGTGGAAGTAGGACTGAAAACTGGGG14980.11975167116734697No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14900.1191121428834092No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC14790.11823279149299477No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13900.1111180393341871No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13760.109998864837296No Hit
CACTATGATAGTCTCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGACAT13560.10840004412745159No Hit
ACATATTACTGTCAACACTATGATAGTCTCCCTCGGACGTTCGGCCAAGG12950.10352364096242611No Hit
ATCATAGTGTTGACAGTAATATGTTGCAATGTCTTCAGGCTGCAGGCTGC12930.10336375889144168No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12780.10216464335905835No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT12520.10008617643626062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22650.066.783964
ATCAACG23750.063.994093
CAACGCA23850.063.423755
AACGCAG24650.061.321226
CGCAGAG29450.051.0780948
GCAGAGT33450.044.97019
GTATCAA38150.040.462131
GCAGCGC7650.039.514968
GTACATG29450.035.7599641
TATCAAC43200.035.1819342
TACATGG29550.034.1264842
CAGCGCT7850.033.9238979
ACATGGG30350.033.2269443
GGCAGCG8550.031.9883027
ACGCAGA54250.027.8607797
GTACACG12350.027.4127065
GTATATA6750.026.715651
CATGGGA19000.026.5378324
TACACGG13050.026.4751956
ATAGCCG18950.026.2067768
Produced by FastQC (version 0.11.5)