FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250922
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTACTGTGTGAGGTTGGGTGGAAGTAGGACTGAAAACTGGGGCCA35940.28730808155904203No Hit
GTATTACTGTGTGAGGTTGGGTGGAAGTAGGACTGAAAACTGGGGCCAGG32470.2595685422432414No Hit
GTAATACATAGCCGTGTCCTCGGCTCGCAGGCTCCTCATGTGCAGATACA30920.24717768174194713No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT26750.21384226994169098No Hit
CAGTAATACATAGCCGTGTCCTCGGCTCGCAGGCTCCTCATGTGCAGATA22120.17682957050879272No Hit
TATAAAAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT21770.17403163426656498No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20640.16499829725594403No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC19770.1580434271681208No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19720.15764372199065968No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18310.14637203598625656No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17590.14061628143081664No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17310.13837793243703445No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC17010.1359797013722678No Hit
CTATGATAGTCTCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGACATCA16660.1331817651300401No Hit
ATATTACTGTCAACACTATGATAGTCTCCCTCGGACGTTCGGCCAAGGGA16450.13150300338470344No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15750.12590713090024797No Hit
GTGTGAGGTTGGGTGGAAGTAGGACTGAAAACTGGGGCCAGGGAATCCTG14390.11503515007330593No Hit
GTACATGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGT14320.11447556282486039No Hit
GCTATGTATTACTGTGTGAGGTTGGGTGGAAGTAGGACTGAAAACTGGGG13980.11175756761812487No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13800.1103186289792649No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13580.10855992619843602No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13470.1076805748080216No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13340.10664134134662275No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12850.10272423060750391No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC12780.10216464335905835No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12590.10064576368470615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13600.059.7598844
CAACGCA13650.059.545755
AACGCAG15350.054.3568886
ATCAACG14950.054.35483
CGCAGAG19100.043.6847238
GCAGAGT23300.035.5015149
TACATGG27300.034.5071682
GCAGCGC7850.033.9029168
GTACATG28350.033.262411
ACATGGG29150.032.070483
CAGCGCT8100.031.9685139
GGCAGCG8150.030.8898227
CATGGGA17650.027.7098644
GTATCAA29600.027.4803451
GTAATAC22300.025.7979873
GTACACG13350.024.2457835
TATCAAC34100.024.0409222
TACACGG14100.022.9561146
TAATACA27700.022.849314
ACGGGGT14400.022.4778619
Produced by FastQC (version 0.11.5)