FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968199
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATAAAGGAGACAGTAATACACGGCCGAGTCGTCAGATCTCAGGCTGC33450.3454868265718101No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30890.3190459812497224No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCAGTGAAAGTCTCCTGCA30340.31336533088755514No Hit
CCTTTATAACAGTGGCCCTACACACTACTTTGACTACTGGGGCCAGGGAA27380.28279310348389125No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26250.27112194910343845No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA25990.2684365507504139No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25920.26771355888613807No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC24270.25067160779963626No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT23910.24695336392621764No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA23620.2439581119170749No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22960.23714133148247415No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22770.23517892499372545No Hit
CTCTGGGACAACACAGCTCGCACAGAAGTTCCGGGGCAGATTCACACTTG22660.234042794921292No Hit
GCCTACATGGAACTGAGCAGCCTGAGATCTGACGACTCGGCCGTGTATTA22520.23259681119274034No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC20080.2073953804951255No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG18540.19148955948105711No Hit
ACATGGAACTGAGCAGCCTGAGATCTGACGACTCGGCCGTGTATTACTGT18460.19066328306474187No Hit
GTGTAGGGCCACTGTTATAAAGGAGACAGTAATACACGGCCGAGTCGTCA18080.18673847008724445No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG17530.1810578197250772No Hit
GTCTCCAGCCACCCTCTCTGCGTCTCCAGGGGAAAGAGCCACCCTCTCCT17180.177442860403698No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16700.17248520190580655No Hit
CCCCTGAACAAGGGCTTGAGTGGATGGGAGGAATCATCCCTCTCTCTGGG16580.1712457872813337No Hit
GTAATACACGGCCGAGTCGTCAGATCTCAGGCTGCTCAGTTCCATGTAGG16360.1689735271364668No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCCTTGTGGTGGCAGCAG16000.1652552832630482No Hit
TTATAACAGTGGCCCTACACACTACTTTGACTACTGGGGCCAGGGAACCC15770.16287973856614188No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15740.16256988491002367No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15580.16091733207739317No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC15230.157302372756014No Hit
CATAACAACCACATTCCTCCTCTGAAGAAGCCCCTGGGAGCACAGCTCAT15220.1571990882039746No Hit
GTATTACTGTCTCCTTTATAACAGTGGCCCTACACACTACTTTGACTACT15000.15492682805910768No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT14960.15451368985095007No Hit
GTGTATTACTGTCTCCTTTATAACAGTGGCCCTACACACTACTTTGACTA14940.15430712074687125No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14860.153480844330556No Hit
GCCCTACACACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTC14470.14945274680101922No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA14210.14676734844799466No Hit
CATGTAGGCTGTGCTCGAGGATTCGTCCGCGACAAGTGTGAATCTGCCCC14090.1455279338235218No Hit
ACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCC13980.14439180375108837No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13450.13891772249299988No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG13320.13757502331648763No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA13020.13447648675530546No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12870.1329272184747144No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12680.1309648119859657No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12490.12900240549721698No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12470.12879583639313819No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAACGACTTAGCCTGGTACCAAC12400.12807284452886236No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC12160.12559401527991662No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG12130.12528416162379843No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT12070.12466445431156198No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12040.12435460065544376No Hit
ACCATGGACTGGACCTGGAGGTTCCTCCTTGTGGTGGCAGCAGCTACAGG12010.12404474699932555No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT11890.1228053323748527No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC11810.12197905595853745No Hit
CATCTATGCTGCATCCACCAGGGCCACCGGTATCCCAGCCAGGTTCAGTG11790.12177248685445864No Hit
TTATTACTGTCAGCAATATAATAACTGGCTGTGGACGTTCGGCCGAGGGA11780.12166920230241923No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG11760.12146263319834041No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG11710.1209462104381434No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11680.12063635678202518No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT11640.12022321857386757No Hit
CTGTTATAAAGGAGACAGTAATACACGGCCGAGTCGTCAGATCTCAGGCT11520.11898380394939471No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCGGTGGCCCTGGT11510.11888051939735529No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA11490.11867395029327649No Hit
GTCCCAGAGAGAGGGATGATTCCTCCCATCCACTCAAGCCCTTGTTCAGG11460.11836409663715827No Hit
ATATAATAACTGGCTGTGGACGTTCGGCCGAGGGACCAAGGTGGAAGTCA11410.11784767387696124No Hit
GTTCCATGTAGGCTGTGCTCGAGGATTCGTCCGCGACAAGTGTGAATCTG11190.11557541373209435No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11130.1149557064198579No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10940.1129932999311092No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC10870.11227030806683336No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10760.11113417799439992No Hit
AGCTACAGGTGTCCAGTCCCAGGTGCAACTGGTGCAGTCTGGGGCTGAGG10760.11113417799439992No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC10580.10927505605769063No Hit
GCTACAGGTGTCCAGTCCCAGGTGCAACTGGTGCAGTCTGGGGCTGAGGT10580.10927505605769063No Hit
GTTATTATATTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC10470.10813892598525716No Hit
TATAATAACTGGCTGTGGACGTTCGGCCGAGGGACCAAGGTGGAAGTCAA10460.10803564143321776No Hit
GCCTGAGATCTGACGACTCGGCCGTGTATTACTGTCTCCTTTATAACAGT10270.10607323494446906No Hit
CAGTAATACACGGCCGAGTCGTCAGATCTCAGGCTGCTCAGTTCCATGTA10020.10349112114348394No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9960.1028714138312475No Hit
GTTATCAACTGGGTGCGACAGACCCCTGAACAAGGGCTTGAGTGGATGGG9950.1027681292792081No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9950.1027681292792081No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC9870.10194185286289287No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA9860.10183856831085344No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA9830.10152871465473523No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC9710.10028930003026237No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)