FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968199
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATAAAGGAGACAGTAATACACGGCCGAGTCGTCAGATCTCAGGCTGC29650.3062386967968362No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCAGTGAAAGTCTCCTGCA29540.3051025667244027No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT28270.2919854286153983No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26770.2764927458094875No Hit
CCTTTATAACAGTGGCCCTACACACTACTTTGACTACTGGGGCCAGGGAA25750.2659577215014682No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22850.23600520141004072No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA22560.23300994940089795No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC22530.23270009574477973No Hit
GCCTACATGGAACTGAGCAGCCTGAGATCTGACGACTCGGCCGTGTATTA21250.21947967308373587No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT21240.21937638853169647No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20920.2160712828664355No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20850.21534829100215966No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20400.21070048616038647No Hit
CTCTGGGACAACACAGCTCGCACAGAAGTTCCGGGGCAGATTCACACTTG20010.20667238863084966No Hit
GTGTAGGGCCACTGTTATAAAGGAGACAGTAATACACGGCCGAGTCGTCA17910.1849826327025746No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC17620.18198738069343182No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG17340.1790954132363285No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG17050.17610016122718572No Hit
GTCTCCAGCCACCCTCTCTGCGTCTCCAGGGGAAAGAGCCACCCTCTCCT16390.169283380792585No Hit
GTAATACACGGCCGAGTCGTCAGATCTCAGGCTGCTCAGTTCCATGTAGG16160.1669078360956787No Hit
ACATGGAACTGAGCAGCCTGAGATCTGACGACTCGGCCGTGTATTACTGT15770.16287973856614188No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA15460.15967791745292031No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15360.15864507193252628No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15160.15657938089173817No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT14560.15038230776937386No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14440.14914289314490098No Hit
GTGTATTACTGTCTCCTTTATAACAGTGGCCCTACACACTACTTTGACTA14420.1489363240408222No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14420.1489363240408222No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCCTTGTGGTGGCAGCAG14310.14780019396838873No Hit
TTATAACAGTGGCCCTACACACTACTTTGACTACTGGGGCCAGGGAACCC14240.14707720210411288No Hit
GCCCTACACACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTC14130.14594107203167944No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14070.145321364719443No Hit
CCCCTGAACAAGGGCTTGAGTGGATGGGAGGAATCATCCCTCTCTCTGGG14040.1450115110633248No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13900.14356552733477312No Hit
GTATTACTGTCTCCTTTATAACAGTGGCCCTACACACTACTTTGACTACT13290.13726516966036942No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC13030.1345797713073449No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12980.13406334854714785No Hit
CATAACAACCACATTCCTCCTCTGAAGAAGCCCCTGGGAGCACAGCTCAT12590.13003525101761104No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12590.13003525101761104No Hit
CATGTAGGCTGTGCTCGAGGATTCGTCCGCGACAAGTGTGAATCTGCCCC12470.12879583639313819No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG12420.12827941363294115No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11870.12259876327077389No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCGGTGGCCCTGGT11860.12249547871873447No Hit
ACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCC11850.12239219416669508No Hit
GTCCCAGAGAGAGGGATGATTCCTCCCATCCACTCAAGCCCTTGTTCAGG11760.12146263319834041No Hit
ATATAATAACTGGCTGTGGACGTTCGGCCGAGGGACCAAGGTGGAAGTCA11700.120842925886104No Hit
GTTATTATATTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC11610.11991336491774934No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG11580.11960351126163113No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT11500.11877723484531588No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11460.11836409663715827No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA11430.11805424298104006No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11410.11784767387696124No Hit
GTTCCATGTAGGCTGTGCTCGAGGATTCGTCCGCGACAAGTGTGAATCTG11120.1148524218678185No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAACGACTTAGCCTGGTACCAAC11090.1145425682117003No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG11020.11381957634742444No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT11010.11371629179538505No Hit
TTATTACTGTCAGCAATATAATAACTGGCTGTGGACGTTCGGCCGAGGGA10970.11330315358722741No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC10950.1130965844831486No Hit
CATCTATGCTGCATCCACCAGGGCCACCGGTATCCCAGCCAGGTTCAGTG10950.1130965844831486No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC10810.11165060075459694No Hit
CTGTTATAAAGGAGACAGTAATACACGGCCGAGTCGTCAGATCTCAGGCT10680.11030790157808468No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10630.10979147881788764No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA10520.10865534874545418No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10480.10824221053729657No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC10460.10803564143321776No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA10290.10627980404854787No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10280.10617651949650847No Hit
GTTATCAACTGGGTGCGACAGACCCCTGAACAAGGGCTTGAGTGGATGGG9870.10194185286289287No Hit
TATAATAACTGGCTGTGGACGTTCGGCCGAGGGACCAAGGTGGAAGTCAA9780.1010122918945382No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10250.061.8207551
ATCAACG10300.060.1224563
TCAACGC10550.058.6977544
CAACGCA11700.052.9283145
AACGCAG12750.047.9923556
GAATACG1751.8189894E-1245.2499359
GGTATCA4300.045.213781
GTGCTAG2600.038.7730671
CAGCGCT7050.035.738999
GCAGAGT17100.035.362799
GTACATG20250.032.003171
GGCAGCG7950.031.6930647
ACATGGG21250.030.4971393
TCTACAC6850.030.484773
GTACAAT2151.9344952E-830.1425191
GCAGCGC8450.029.8177368
CGCAGAG20850.029.3478438
GTATGTA14250.028.8028531
CAGGGCA10950.027.6191714
TATGTAC14200.026.87592
Produced by FastQC (version 0.11.5)