FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897096
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATTAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG23340.260172824313117No Hit
AATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTCTGTTCGGCGG18850.21012243951594925No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16280.1814744464360559No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC12030.13409936060354744No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG11480.12796846714286989No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC11440.12752258398209335No Hit
GTAATAATCAGCGACCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG11430.12741111319189918No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT10670.11893933313714473No Hit
GACCTAGGACGGTCAGGTAGGTCCCTCCGCCGAACAGACCATTCAGGCTG9500.10589725068443066No Hit
GTAATACACGGCCGTGTCCTCAGATCTGAGGCTGCTCACCTCCATGTACA9500.10589725068443066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17650.069.8073351
TCAACGC19750.060.513414
ATCAACG20050.059.6112983
CAACGCA20250.059.019255
AACGCAG21250.056.241876
TATCAAC22350.054.1210982
ACGCAGA24000.049.494757
CGCAGAG25100.047.325668
GCAGAGT34950.033.7818269
GGTATCA18900.030.1172281
ATACACG6950.030.0416554
TACACGG6850.029.4291765
ATATAGG4700.024.5285451
GTAATAC8650.024.1563641
TACGGGC1500.001556552623.9974568
GTACATG27000.023.4838121
GCCTCGT2852.9728835E-722.7344327
GTGTAGA5400.022.6832261
TACATGG27150.022.2763652
GTACGGA1952.7406932E-422.152736
Produced by FastQC (version 0.11.5)