FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775693
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTAGTCCATTCACTTTCGGCCCTGGGACCAAAGTCGATGTCA27590.3556819514936966No Hit
CTATAATACTGTTGACACCAATAAGTTGCAGAATCTTCAGGCTGCAGGCT23050.2971536419691811No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15560.20059482295186368No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15530.2002080720078691No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15500.1998213210638745No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14930.1924730531279772No Hit
CTATTCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCA14750.19015254746400959No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14080.18151510971479698No Hit
GATCAGCACTGAGCACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGG13710.17674518140553028No Hit
GTCTGAGAACTGAGGACACCGCCTTCTATTACTGTGGCAAGGGTTTAGCA12710.1638534832723771No Hit
GTTCTCAGACTGTTCATTAGCAGATACAGGGAGTTTTTGCTGTTGTCTCT12220.15753655118713203No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11910.15354012476585455No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11850.15276662287786533No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11340.14619185682995722No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11260.14516052097930496No Hit
GTCTGAATAGTATGTATAATCACCATCCCGACTAATAACAGAGAGCCACT11080.14284001531533738No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11060.1425821813526743No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10250.13213990586482025No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9910.12775672849954814No Hit
GTAATAGAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTAGCAGATACA9910.12775672849954814No Hit
CTATTACTGTGGCAAGGGTTTAGCAGCAGCTGGTCGCCTTTCGACCGCCC9630.12414705302226527No Hit
ATTCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAA9470.12208438132096074No Hit
AATCAAGGGCGGTCGAAAGGCGACCAGCTGCTGCTAAACCCTTGCCACAG9300.11989279263832471No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9280.11963495867566164No Hit
ACTCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCCAGGTTCAGTG9190.11847470584367785No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8930.11512286432905801No Hit
CAGTAATAGAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTAGCAGATA8890.1146071964037319No Hit
TTTAAGAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGCCG8820.11370477753441116No Hit
ATAGTAGTCCATTCACTTTCGGCCCTGGGACCAAAGTCGATGTCAAACGA8750.11280235866509045No Hit
ACTATAATACTGTTGACACCAATAAGTTGCAGAATCTTCAGGCTGCAGGC8410.10841918129981835No Hit
TTCTATTACTGTGGCAAGGGTTTAGCAGCAGCTGGTCGCCTTTCGACCGC7990.10300466808389401No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG7910.10197333223324176No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7890.10171549827057869No Hit
GTTCATTAGCAGATACAGGGAGTTTTTGCTGTTGTCTCTGGAGATGGTGA7870.10145766430791563No Hit
GGTCTGGAGTGGCTCTCTGTTATTAGTCGGGATGGTGATTATACATACTA7800.10055524543859491No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA7770.1001684944946003No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13050.065.8209841
TCAACGC15700.053.6528554
ATCAACG15900.052.9779783
CAACGCA16200.051.9969025
TATCAAC16700.050.47592
AACGCAG17250.048.8318756
TAACACG450.00990778147.9971474
TAGTACG450.00990778147.9971474
CGCAGAG19750.043.015168
CGTCTAT700.00107960141.246761
CAGCGCT4100.040.387849
GCAGAGT22300.038.0963869
ACGCAGA22700.037.1079257
CGTACAC850.002837043333.8803373
GTACATG17150.033.6708261
GTATTAT15500.033.063941
TTATAGT14850.031.9980954
TACATGG17700.031.7494352
ACATGGG18300.031.4735343
GCAGCGC5500.031.4163118
Produced by FastQC (version 0.11.5)