FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775693
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTAGTCCATTCACTTTCGGCCCTGGGACCAAAGTCGATGTCA27390.35310361186706596No Hit
CTATAATACTGTTGACACCAATAAGTTGCAGAATCTTCAGGCTGCAGGCT19330.24919652491385125No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14460.18641395500539518No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13220.17042824932028522No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12890.16617398893634466No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12440.16037272477642572No Hit
CTATTCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCA12350.15921247194444194No Hit
GATCAGCACTGAGCACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGG12250.1579233021311266No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12230.15766546816846355No Hit
GTCTGAGAACTGAGGACACCGCCTTCTATTACTGTGGCAAGGGTTTAGCA11000.14180867946468512No Hit
GTTCTCAGACTGTTCATTAGCAGATACAGGGAGTTTTTGCTGTTGTCTCT10970.14142192852069055No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10740.1384568379500653No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10390.13394474360346167No Hit
GTCTGAATAGTATGTATAATCACCATCCCGACTAATAACAGAGAGCCACT10320.13304232473414096No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9940.12814347944354276No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9660.12453380396625985No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8720.11241560772109584No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8190.10558300771052465No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8000.10313358506522555No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7810.10068416241992643No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9750.053.946871
ATCAACG9550.050.504183
TCAACGC9800.049.2158134
CCTATCG450.00991235947.991538
CAACGCA10550.046.39945
AACGCAG11250.042.8724376
TATCAAC12050.041.8183482
CGCAGAG12700.036.2770658
TACACGG7050.033.6961826
CAGCGCT3900.033.2249079
TAGACTG2757.2759576E-1231.412645
CTATAAT10550.030.7332211
GTATTAT15600.030.4836771
ATTATAG15150.029.9353143
ACACGGG7950.029.8815217
GCAGAGT15750.029.2519849
GTACACG8000.028.794925
TTATAGT15250.028.794924
ATAGTAG15600.028.6103366
GTACATG18200.028.5042171
Produced by FastQC (version 0.11.5)