FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810395
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT26650.32885197959019985No Hit
GTGTGTACCTAGCATGCAGTAATAAAGTCCAACATCCTCAGCCTCCACCC20130.2483973864596894No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19530.24099358954583877No Hit
TTATTACTGCATGCTAGGTACACACTGGCCTCCGATGTACACTTTTGGCC18530.2286539280227543No Hit
GTATGTGAGATCTCCACTAGCACTAATACTTGAGACCCACTCCAGCCCCT18100.22334787356782806No Hit
GGTACACACTGGCCTCCGATGTACACTTTTGGCCAGGGGACCAAGCTCGA17130.21137840189043616No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15900.1962006182170423No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13480.166338637331178No Hit
GAAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAAAAACA13090.1615261693371751No Hit
AGGTACACACTGGCCTCCGATGTACACTTTTGGCCAGGGGACCAAGCTCG12730.15708389118886468No Hit
CGTATACAGTGATGGAAACACCTACTTGAATTGGTATCAGCAGAGGCCAG12390.152888406271016No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12200.15054387058162996No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11980.14782914504655137No Hit
GTGATGGAAACACCTACTTGAATTGGTATCAGCAGAGGCCAGGCCAATCT11810.145731402587627No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT11800.14560800597239618No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11800.14560800597239618No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11550.14252309059162507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11460.14141252105454746No Hit
CTCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCCTCTCCAGAGA11350.1400551582870082No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11280.13919138198039227No Hit
GTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAA11180.13795741582808382No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA11130.13734043275192961No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11050.13635325983008287No Hit
TCTCTGGAGAGGGTGAACCGGCCCTTCACGGAGTCTGCGTAGTATGTGAG10950.13511929367777442No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTATCAGCAGAGGCC10840.13376193091023514No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA10750.13265136137315753No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT10660.13154079183607992No Hit
CTCCAGAGACAATTCCATGAACACGCTGTTTTTGCAAATGAACAGCCTGA10150.12524756445930688No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC10120.12487737461361434No Hit
GTCTGGGACCCCAGAGTCCCGGCTAGAAACCTTATAAATTAGGCGCCTTG10080.12438378815269097No Hit
TTTATAAGGTTTCTAGCCGGGACTCTGGGGTCCCAGACAGATTCAGCGGC10040.12389020169176759No Hit
GGTATCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTT9920.12240944230899747No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9670.11932452692822634No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC9400.11599281831699355No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG9130.11266110970576076No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9070.11192073001437569No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT8910.1099463841706822No Hit
GCCTGGGGGATCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA8880.10957619432498966No Hit
GTAATAAAGTCCAACATCCTCAGCCTCCACCCTGCTGATCGTCAGTGTGA8880.10957619432498966No Hit
CTGTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCC8730.107725245096527No Hit
GATTTCACACTGACGATCAGCAGGGTGGAGGCTGAGGATGTTGGACTTTA8730.107725245096527No Hit
CAGTAATAAAGTCCAACATCCTCAGCCTCCACCCTGCTGATCGTCAGTGT8700.10735505525083448No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8690.10723165863560362No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA8630.10649127894421856No Hit
TTCCATCACTGTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCC8600.10612108909852604No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8510.10501051956144843No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA8490.10476372633098673No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC8450.10427013987006335No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA8220.10143201771975394No Hit
GTACACACTGGCCTCCGATGTACACTTTTGGCCAGGGGACCAAGCTCGAG8210.1013086211045231No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGG8180.10093843125883056No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8170.10081503464359973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)