FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577709
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC19770.34221381352895663No Hit
GTAATAGACGGCCGTGTCGTCAGATGTCAGGCTGGTCACCTCCATATAGG18730.32421167058155576No Hit
GCCTATATGGAGGTGACCAGCCTGACATCTGACGACACGGCCGTCTATTA17230.29824704133049684No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA15430.2670894862292261No Hit
GTCTATTACTGTGCGAGGGATAAGAAAGGGACTATAGTAACTACGACGGG14170.24527919765833664No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGCGCTGCTG13350.23108520033442442No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG12220.21152517963196005No Hit
CCATATAGGCTGTGCTGATGGACGTGTCCCTGGTCAGGACGACCCTGCCC11850.20512057108336548No Hit
ACTATATTCTCTGGGTGCGACAGGCCCCTGGACAACGGCTTGAGTACATG11430.19785047489306898No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC10160.1758670887938391No Hit
CTCCTGGACAGTCGATCACCATCTACTGCACTGGAACCAGCAGTGACATT10100.17482850362379676No Hit
GATTATTACTGTAGCTCATATACAAGCAGCAGCGCTGTGGTATTCGGCGG9770.1691162851885638No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT9720.1682507975468618No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT9700.16790460249018105No Hit
CATATAGGCTGTGCTGATGGACGTGTCCCTGGTCAGGACGACCCTGCCCC9150.15838423843145943No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCAC9030.15630706809137473No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9000.15578777550635353No Hit
TTCTTATCCCTCGCACAGTAATAGACGGCCGTGTCGTCAGATGTCAGGCT8970.15526848292133236No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA8730.15111414224116296No Hit
ATATGGAGGTGACCAGCCTGACATCTGACGACACGGCCGTCTATTACTGT8000.13847802267231427No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7670.13276580423708131No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG7670.13276580423708131No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCACAGCGCTGCTGC7500.12982314625529462No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT7380.12774597591520992No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT7350.12722668333018874No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7240.1253226105184444No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7230.12514951299010402No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC7150.12376473276338087No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC7080.12255305006499811No Hit
GGATAAGAAAGGGACTATAGTAACTACGACGGGTCTCTACGGTATGGACG6980.12082207478159418No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6780.11736012421478634No Hit
CTACTATATTCTCTGGGTGCGACAGGCCCCTGGACAACGGCTTGAGTACA6760.11701392915810555No Hit
GGTTATAACTATGTCTCCTGGTACCAGCAACACCCAGGCAAAGTCCCCAA6720.11632153904474397No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGTAGCTCATAT6300.10905144285444747No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTTAGCCTCC6210.10749356509938396No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA6170.10680117498602239No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA6150.10645497992934158No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6130.10610878487266079No Hit
CTGCTATTCCTCACCCTCGTCACTCAGGGCACAGGGTCCTGGGCCCAGTC6120.1059356873443204No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA6070.10507019970261845No Hit
CCCTAATAATGGAGCCACAATCTCTGCACAGAAGTTTCGGGGCAGGGTCG6030.10437780958925688No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT5780.10005037138074706No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC350.00366817161.7001578
GAATACG1900.045.4632729
AAGCGCT1351.0915755E-842.6568959
TATAGCG700.001094081841.133444
GTATCAA9350.038.530541
GTGTCGC750.001535887138.3912059
ATCAACG9200.038.3390463
TCAACGC9550.036.1801954
GTGCTAG3200.036.0260541
GAAGCGC2053.1468517E-1035.1139078
TATCAAC10600.033.9544872
CAACGCA10350.033.383665
AACGCAG10600.032.5963066
CGCAGAG11450.030.1764958
TGCTAGA3600.029.9931282
TCTACAC4700.029.0997183
GGTATCA5450.029.0852551
GGAAGCG2502.6357156E-928.7934067
TACTATC1358.423029E-426.660562
TACACAG7100.026.360165
Produced by FastQC (version 0.11.5)